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Our Abpromise guarantee covers the use of ab6632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/500 - 1/2000.|
|IHC-Fr||1/1000 - 1/5000.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ab6632 at 1/10 dilution staining human renal carcinoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was stained with antibody for 12 hours and then detected using a biotinylated donkey anti-mouse IgG antibody.
This image is courtesy of an Abreview submitted on 3 March 2006.
ab6632 staining CD62P in thrombin activated human platelets by Flow Cytometry analysis.
ab6632 staining CD62P in rhesus monkey myometrium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 2% serum for 20 minutes at 25°C. Samples were incubated with primary antibody at 1/500 dilution for 16 hours at 4°C. A biotin conjugated horse polyclonal was used as the secondary antibody at 1/200 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"