• Product name
    Anti-CD62P antibody [AK-6]
    See all CD62P primary antibodies
  • Description
    Mouse monoclonal [AK-6] to CD62P
  • Host species
  • Specificity
    MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Rhesus monkey
  • Positive control
    • IHC-P: Human renal carcinoma tissue. IHC-Fr: Rhesus monkey myometrium tissue. Flow: Thrombin activated human platelets.



Our Abpromise guarantee covers the use of ab6632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/500 - 1/2000.
IHC-Fr 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


  • Function
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD62 antibody
    • CD62 antibody
    • CD62 antigen-like family member P antibody
    • CD62P antibody
    • GMP 140 antibody
    • GMP-140 antibody
    • GMP140 antibody
    • GMRP antibody
    • Granule membrane protein 140 antibody
    • Granulocyte membrane protein antibody
    • GRMP antibody
    • LECAM 3 antibody
    • LECAM3 antibody
    • Leukocyte endothelial cell adhesion molecule 3 antibody
    • Leukocyte-endothelial cell adhesion molecule 3 antibody
    • LYAM3_HUMAN antibody
    • P Selectin antibody
    • P-selectin antibody
    • PADGEM antibody
    • Platelet activation dependent granule-external membrane protein antibody
    • Platelet alpha granule membrane protein antibody
    • PSEL antibody
    • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
    • SELP antibody
    see all


  • ab6632 at 1/10 dilution staining human renal carcinoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was stained with antibody for 12 hours and then detected using a biotinylated donkey anti-mouse IgG antibody.

    This image is courtesy of an Abreview submitted on 3 March 2006.

    See Abreview

  • ab6632 staining CD62P in thrombin activated human platelets by Flow Cytometry analysis.

  • ab6632 staining CD62P in rhesus monkey myometrium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 2% serum for 20 minutes at 25°C. Samples were incubated with primary antibody at 1/500 dilution for 16 hours at 4°C. A biotin conjugated horse polyclonal was used as the secondary antibody at 1/200 dilution.

    See Abreview


This product has been referenced in:
  • Farinazzo A  et al. Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis. Sci Rep 8:7473 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29748664) »
  • Gillies PJ  et al. Demonstration of P-selectin expression and potential function in human corneal epithelial cells. Exp Eye Res 176:196-206 (2018). Read more (PubMed: 30025918) »
See all 7 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Rhesus monkey Tissue sections (myometrium)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 22 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (nasal mucosa)
nasal mucosa
Blocking step
H2O2 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Michael Könnecke

Verified customer

Submitted Jan 07 2013


Thank you for contacting us.

ab6632 has yet not been tested in ELISA application; this however doesn't mean this product is not suitable, the product might be suitable however we do not have data to confirm it.

On the other hand product ab78440 is tested in ELISA and is fully guaranteed;


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Thank you for your enquiry regarding ab6632. Unfortunately this antibody has not been epitope mapped so I cannot tell you exactly which amino acids it recognises. However this clone, AK-6, was studied as part of the Adhesion Structures section of Leucocyte Typing V, Volume 2. In this report the epitope for this clone is localised to the SCR domain of CD62P. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Mar 03 2006


Thank you for your enquiry. This antibody is specific for CD62P. To our knowledge it has not been tested for cross-reactivity with E-selectin (CD62E). As discussed in the reference given below, this antibody was raised against a human platelet glycoprotein which was confirmed to be platelet GMP-140 (or CD62P). The exact epitope recognised by this antibody has yet to be determined. Skinner, M.P. et al. (1989). Characterisation of human platelet GMP-140 as a heparin-binding protein. Biochem Biophys Res Commun. 164(3):1373-1376. We would usually recommend a concentration of 1-10ug/ml for the use of antibodies in Western blotting. Based on this estimate you may need to increase the concentration of antibody used further (i.e. to 1:100). We hope that this information is of assistance.

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This antibody has been shown to cross-react with human and Rhesus monkey. Other species have not been tested. This antibody is suitable for use on formalin fixed tissue.

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