- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-CD62P antibody [AK-6]
See all CD62P primary antibodies -
DescriptionMouse monoclonal [AK-6] to CD62P
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Host speciesMouse
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SpecificityMCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
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Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details
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Species reactivityReacts with: Mouse, Human, Rhesus monkey
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Positive control
- IHC-P: Human renal carcinoma tissue. IHC-Fr: Rhesus monkey myometrium tissue. Flow: Thrombin activated human platelets.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.60
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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ClonalityMonoclonal
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Clone numberAK-6
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab6632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/500 - 1/2000. | |
| IHC-Fr | 1/1000 - 1/5000. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
Target
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FunctionCa(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
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Tissue specificityStored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
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Involvement in diseaseDefects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
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Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 6403 Human
- Entrez Gene: 20344 Mouse
- Omim: 173610 Human
- SwissProt: P16109 Human
- SwissProt: Q01102 Mouse
- Unigene: 73800 Human
- Unigene: 3337 Mouse
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Alternative names
- Antigen CD62 antibody
- CD62 antibody
- CD62 antigen-like family member P antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)](https://www.abcam.com/ps/products/6/ab6632/Images/ab6632_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)ab6632 at 1/10 dilution staining human renal carcinoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was stained with antibody for 12 hours and then detected using a biotinylated donkey anti-mouse IgG antibody.
This image is courtesy of an Abreview submitted on 3 March 2006.
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![Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)](https://www.abcam.com/ps/products/6/ab6632/Images/ab6632_2.jpg)
Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)ab6632 staining CD62P in thrombin activated human platelets by Flow Cytometry analysis.
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![Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)](https://www.abcam.com/ps/products/6/ab6632/Images/ab6632-305766-ab6632.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)This image is courtesy of an anonymous Abreviewab6632 staining CD62P in rhesus monkey myometrium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 2% serum for 20 minutes at 25°C. Samples were incubated with primary antibody at 1/500 dilution for 16 hours at 4°C. A biotin conjugated horse polyclonal was used as the secondary antibody at 1/200 dilution.
Protocols
References
This product has been referenced in:
- Farinazzo A et al. Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis. Sci Rep 8:7473 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29748664) »
- Ma R et al. Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis. Sci Rep 7:4978 (2017). Read more (PubMed: 28694452) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"