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  1. Link

    cd62p-antibody-ak-6-ab6632.pdf

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Recombinant

Recombinant Anti-CD62P antibody [AK-6] (ab6632)

  • Datasheet
  • SDS
Reviews (5)Q&A (5)References (15)

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Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)
  • Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD62P antibody [AK-6] (ab6632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AK-6] to CD62P
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD62P antibody [AK-6]
    See all CD62P primary antibodies
  • Description

    Mouse monoclonal [AK-6] to CD62P
  • Host species

    Mouse
  • Specificity

    MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human whole blood Flow Cyt: Human whole blood IHC-P: Human bone marrow and human colon tissue
  • General notes

    This product has switched from a hybridoma to recombinant production method on 22nd July 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    AK-6
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Thrombocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-CD62P antibody [AK-6] - BSA and Azide free (ab252267)
    • FITC Anti-CD62P antibody [AK-6] (ab33279)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human CD62P protein (Fc Chimera) (ab83583)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 0.2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (2)
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 10 µg/ml.
IHC-Fr (3)
Use a concentration of 0.01 µg/ml.
Notes
Flow Cyt
Use a concentration of 0.2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 10 µg/ml.
IHC-Fr
Use a concentration of 0.01 µg/ml.

Target

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P16109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6403 Human
    • Omim: 173610 Human
    • SwissProt: P16109 Human
    • Unigene: 73800 Human
    • Alternative names

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)
      Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)

      Flow cytometry staining of human whole blood with ab6632 (right) or mouse IgG1κ (ab170190) isotype (left). Red blood cells of 200 µl blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab6632) or mouse IgG1κ (ab170190) isotype (1x106 in 100 µl; at 0.2 µg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor®488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD42a PE. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on alive neutrophils.

       

    • Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)
      Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)

      IHC image of CD62P staining in a section of frozen normal human colon* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab6632, 0.01ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)

      IHC image of CD62P staining in a section of formalin-fixed paraffin-embedded normal human bone marrow* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6632, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)

      IHC image of CD62P staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6632, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunocytochemistry/ Immunofluorescence - Anti-CD62P antibody [AK-6] (ab6632)
      Immunocytochemistry/ Immunofluorescence - Anti-CD62P antibody [AK-6] (ab6632)

      ab6632 staining CD62P in Human whole blood. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6632 at 10μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (15)

    Publishing research using ab6632? Please let us know so that we can cite the reference in this datasheet.

    ab6632 has been referenced in 15 publications.

    • Sun C  et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
    • Zhou P  et al. Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion. EBioMedicine 53:102671 (2020). PubMed: 32114386
    • Zhang K  et al. Activation of brain endothelium by Escherichia coli K1 virulence factor cglD promotes polymorphonuclear leukocyte transendothelial migration. Med Microbiol Immunol 208:59-68 (2019). PubMed: 30171337
    • Bertrand L  et al. Targeting the HIV-infected brain to improve ischemic stroke outcome. Nat Commun 10:2009 (2019). PubMed: 31043599
    • Barranco R  et al. Immunohistochemical expression of P-selectin, SP-A, HSP70, aquaporin 5, and fibronectin in saltwater drowning and freshwater drowning. Int J Legal Med 133:1461-1467 (2019). PubMed: 31222534
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human brain)
    Permeabilization
    No
    Specification
    Human brain
    Blocking step
    Antibody Diluent with Background Reducing (Dako/Agilent) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 07 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Human brain
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 25 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (myometrium)
    Permeabilization
    No
    Specification
    myometrium
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 22 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (nasal mucosa)
    Specification
    nasal mucosa
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    H2O2 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Michael Könnecke

    Verified customer

    Submitted Jan 07 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 03 2006

    Question

    Inquiry: Since I attend to measure the expression of P-selectin in IL-4 stimulated human umbilical vein endothelial cells by ELISA, I would like to know if the P-Selectin Antibody (AK6 clone, product code 6632) is recommended for this use.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2012

    Answer

    Thank you for contacting us.

    ab6632 has yet not been tested in ELISA application; this however doesn't mean this product is not suitable, the product might be suitable however we do not have data to confirm it.

    On the other hand product ab78440 is tested in ELISA and is fully guaranteed;

    https://www.abcam.com/CD62P-antibody-P8G6-ab78440.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 18 2012

    Question

    No signal in western blots of human platelet lysates compared to previous lot.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I have issued a free of charge replacement.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    Where on the protein does this antibody bind?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 03 2007

    Answer

    Thank you for your enquiry regarding ab6632. Unfortunately this antibody has not been epitope mapped so I cannot tell you exactly which amino acids it recognises. However this clone, AK-6, was studied as part of the Adhesion Structures section of Leucocyte Typing V, Volume 2. In this report the epitope for this clone is localised to the SCR domain of CD62P. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 03 2007

    Question

    I have been using ab6632(Mouse monoclonal to P-Selectin) for microscopy and had good results as I mentioned in my last email. Your support told me I could use thia Ab for WB in either non-reducing or reducing conditions. I have tried non reducing conditions, using the Ab at 1:1000 and 1:250 with both secondary to HRP and AP with no results. I am currently doing a reducing western. I would like to know what specific part of the P-Selectin antigen does this Ab bind to? Secondly, does this cross react with E-Selectin?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2004

    Answer

    Thank you for your enquiry. This antibody is specific for CD62P. To our knowledge it has not been tested for cross-reactivity with E-selectin (CD62E). As discussed in the reference given below, this antibody was raised against a human platelet glycoprotein which was confirmed to be platelet GMP-140 (or CD62P). The exact epitope recognised by this antibody has yet to be determined. Skinner, M.P. et al. (1989). Characterisation of human platelet GMP-140 as a heparin-binding protein. Biochem Biophys Res Commun. 164(3):1373-1376. We would usually recommend a concentration of 1-10ug/ml for the use of antibodies in Western blotting. Based on this estimate you may need to increase the concentration of antibody used further (i.e. to 1:100). We hope that this information is of assistance.

    Read More

    Abcam Scientific Support

    Answered on Dec 13 2004

    Question

    Will CD62P work on other species? On formalin fixed tissue?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2002

    Answer

    This antibody has been shown to cross-react with human and Rhesus monkey. Other species have not been tested. This antibody is suitable for use on formalin fixed tissue.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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