Anti-CD62P antibody [AK-6] (ab6632)

Mouse monoclonal CD62P antibody [AK-6]. Validated in WB, IP, IHC, Flow Cyt and tested in Mouse, Human, Rhesus monkey. Cited in 7 publication(s). Independently reviewed in 5 review(s).

Reviews (5)Q&A (5)References (9)

Overview

  • Product name

    Anti-CD62P antibody [AK-6]
    See all CD62P primary antibodies

  • Description

    Mouse monoclonal [AK-6] to CD62P

  • Host species

    Mouse

  • Specificity

    MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human, Rhesus monkey

  • Positive control

    • IHC-P: Human renal carcinoma tissue. IHC-Fr: Rhesus monkey myometrium tissue. Flow: Thrombin activated human platelets.

Properties

Applications

Our Abpromise guarantee covers the use of ab6632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/500 - 1/2000.
IHC-Fr 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)

      ab6632 at 1/10 dilution staining human renal carcinoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was stained with antibody for 12 hours and then detected using a biotinylated donkey anti-mouse IgG antibody.

      This image is courtesy of an Abreview submitted on 3 March 2006.

      See Abreview

    • Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)
      Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)

      ab6632 staining CD62P in thrombin activated human platelets by Flow Cytometry analysis.

    • Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)
      Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)This image is courtesy of an anonymous Abreview

      ab6632 staining CD62P in rhesus monkey myometrium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 2% serum for 20 minutes at 25°C. Samples were incubated with primary antibody at 1/500 dilution for 16 hours at 4°C. A biotin conjugated horse polyclonal was used as the secondary antibody at 1/200 dilution.

      See Abreview

    References

    This product has been referenced in:

    • Zhang K  et al. Activation of brain endothelium by Escherichia coli K1 virulence factor cglD promotes polymorphonuclear leukocyte transendothelial migration. Med Microbiol Immunol 208:59-68 (2019). Read more (PubMed: 30171337) »
    • Bertrand L  et al. Targeting the HIV-infected brain to improve ischemic stroke outcome. Nat Commun 10:2009 (2019). Read more (PubMed: 31043599) »
    See all 9 Publications for this product

    Publishing research using ab6632? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human brain)
    Permeabilization
    No
    Specification
    Human brain
    Blocking step
    Antibody Diluent with Background Reducing (Dako/Agilent) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 07 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Human brain
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (myometrium)
    Permeabilization
    No
    Specification
    myometrium
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (nasal mucosa)
    Specification
    nasal mucosa
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    H2O2 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Mr. Michael Könnecke

    Verified customer

    Submitted Jan 07 2013

    Question

    Inquiry: Since I attend to measure the expression of P-selectin in IL-4 stimulated human umbilical vein endothelial cells by ELISA, I would like to know if the P-Selectin Antibody (AK6 clone, product code 6632) is recommended for this use.

    Read More
    Answer

    Thank you for contacting us.

    ab6632 has yet not been tested in ELISA application; this however doesn't mean this product is not suitable, the product might be suitable however we do not have data to confirm it.

    On the other hand product ab78440 is tested in ELISA and is fully guaranteed;

    https://www.abcam.com/CD62P-antibody-P8G6-ab78440.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    No signal in western blots of human platelet lysates compared to previous lot.

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I have issued a free of charge replacement.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Where on the protein does this antibody bind?

    Read More
    Answer

    Thank you for your enquiry regarding ab6632. Unfortunately this antibody has not been epitope mapped so I cannot tell you exactly which amino acids it recognises. However this clone, AK-6, was studied as part of the Adhesion Structures section of Leucocyte Typing V, Volume 2. In this report the epitope for this clone is localised to the SCR domain of CD62P. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 03 2006

    Question

    I have been using ab6632(Mouse monoclonal to P-Selectin) for microscopy and had good results as I mentioned in my last email. Your support told me I could use thia Ab for WB in either non-reducing or reducing conditions. I have tried non reducing conditions, using the Ab at 1:1000 and 1:250 with both secondary to HRP and AP with no results. I am currently doing a reducing western. I would like to know what specific part of the P-Selectin antigen does this Ab bind to? Secondly, does this cross react with E-Selectin?

    Read More
    Answer

    Thank you for your enquiry. This antibody is specific for CD62P. To our knowledge it has not been tested for cross-reactivity with E-selectin (CD62E). As discussed in the reference given below, this antibody was raised against a human platelet glycoprotein which was confirmed to be platelet GMP-140 (or CD62P). The exact epitope recognised by this antibody has yet to be determined. Skinner, M.P. et al. (1989). Characterisation of human platelet GMP-140 as a heparin-binding protein. Biochem Biophys Res Commun. 164(3):1373-1376. We would usually recommend a concentration of 1-10ug/ml for the use of antibodies in Western blotting. Based on this estimate you may need to increase the concentration of antibody used further (i.e. to 1:100). We hope that this information is of assistance.

    Read More

    Question

    Will CD62P work on other species? On formalin fixed tissue?

    Read More
    Answer

    This antibody has been shown to cross-react with human and Rhesus monkey. Other species have not been tested. This antibody is suitable for use on formalin fixed tissue.

    Read More

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