Western blot abreview for Anti-CD62P antibody [EPR1444(2)(B)]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bEnd.3)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
bEnd.3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/1000

Secondary antibody

Dilution
1/4000
Name
Non-Abcam antibody was used: Millipore AP132P
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
35 second(s)
Bands
Specific: 100~130 kDa

Additional data

Additional Notes
84669

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Submitted Mar 23 2018

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