Recombinant
RabMAb

Recombinant Anti-CD62P antibody [EPR1444(2)(B)] - BSA and Azide free (ab238918)

Overview

  • Product name

    Anti-CD62P antibody [EPR1444(2)(B)] - BSA and Azide free
    See all CD62P primary antibodies
  • Description

    Rabbit monoclonal [EPR1444(2)(B)] to CD62P - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD62P aa 800 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P16109

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    Ab238918 is the carrier-free version of ab178424. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238918 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
    • Tissue specificity

      Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
    • Involvement in disease

      Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • Sequence similarities

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 9 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human vessel tissue labeling CD62P with ab178424 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178424).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD62P with ab178424 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178424).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab238918 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab238918.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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