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RecombinantRabMAb

Recombinant Anti-CD62P antibody [EPR2146(2)] (ab182135)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)
  • Immunoprecipitation - Anti-CD62P antibody [EPR2146(2)] (ab182135)
  • Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
  • Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
  • Anti-CD62P antibody [EPR2146(2)] (ab182135)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2146(2)] to CD62P
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD62P antibody [EPR2146(2)]
    See all CD62P primary antibodies

  • Description

    Rabbit monoclonal [EPR2146(2)] to CD62P

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, Human placenta and plasma lysates; Human spleen and gliocytoma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
IP
1/30.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
IP
1/30.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)

      Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CD62P with ab182135 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD62P with ab182135 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Immunoprecipitation - Anti-CD62P antibody [EPR2146(2)] (ab182135)

      Western blot analysis of immunoprecipitation pellet from Human plasma immunoprecipitated using ab182135 at 1/30 dilution.  Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

    • Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Anti-CD62P antibody [EPR2146(2)] (ab182135) at 1/10000 dilution + Human plasma lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 91 kDa

    • Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)
      All lanes : Anti-CD62P antibody [EPR2146(2)] (ab182135) at 1/2000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human placenta lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP at 1/1000 dilution

      Predicted band size: 91 kDa

    • Anti-CD62P antibody [EPR2146(2)] (ab182135)
      Anti-CD62P antibody [EPR2146(2)] (ab182135)

    References (1)

    Publishing research using ab182135? Please let us know so that we can cite the reference in this datasheet.

    ab182135 has been referenced in 1 publication.

    • Williams H  et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal 15:421-432 (2021). PubMed: 34080128

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