Overview

  • Product name

    Anti-CD62P antibody [Psel.KO.2.7]
    See all CD62P primary antibodies
  • Description

    Mouse monoclonal [Psel.KO.2.7] to CD62P
  • Host species

    Mouse
  • Specificity

    This antibody recognizes the CD62P cell surface antigen
  • Tested applications

    Suitable for: IP, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD62P. Transfected mouse 300.19 cells

  • Positive control

    • FC: Thrombin activated human platelets IHC-Fr: Human Tonsil
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab54427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.
Flow Cyt 1/25 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen CD62 antibody
    • CD62 antibody
    • CD62 antigen-like family member P antibody
    • CD62P antibody
    • GMP 140 antibody
    • GMP-140 antibody
    • GMP140 antibody
    • GMRP antibody
    • Granule membrane protein 140 antibody
    • Granulocyte membrane protein antibody
    • GRMP antibody
    • LECAM 3 antibody
    • LECAM3 antibody
    • Leukocyte endothelial cell adhesion molecule 3 antibody
    • Leukocyte-endothelial cell adhesion molecule 3 antibody
    • LYAM3_HUMAN antibody
    • P Selectin antibody
    • P-selectin antibody
    • PADGEM antibody
    • Platelet activation dependent granule-external membrane protein antibody
    • Platelet alpha granule membrane protein antibody
    • PSEL antibody
    • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
    • SELP antibody
    see all

Images

  • ab54427, at a 1/25 dilution, staining thrombin activated human platelets

References

This product has been referenced in:

  • Shen DS  et al. Aspirin eugenol ester inhibits agonist-induced platelet aggregation in vitro by regulating PI3K/Akt, MAPK and Sirt 1/CD40L pathways. Eur J Pharmacol 852:1-13 (2019). Read more (PubMed: 30797789) »
  • Li XH  et al. Effect of Platelet-derived P-selectin on Neutrophil Recruitment in a Mouse Model of Sepsis-induced Acute Kidney Injury. Chin Med J (Engl) 130:1694-1699 (2017). Mouse . Read more (PubMed: 28685720) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your reply.

I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

I look forward to your reply and helping resolve this matter.

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Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

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Question
Answer

Yes, you can use ab6947 with ab54427. The antibody that recognizes IgG will recognize all IgG subtypes, whereas the IgG1, will only recognize IgG. Therefore, if you are not intending to use any other mouse primary antibodies at the same time then the IgG antibody will work well, if however you would like to use other mouse antibodies, then I would recommend the IgG1.

Please let me know if there is anything else I can help you with, and if you can provide me the address, I can generate a quote.

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Answer

Thank you for your inquiry. I am happy to confirm that we offer an antibody against P selectin (CD62P) that is tested and guaranteed for horse samples (the prize can be found on the top of the datasheet): ab54427 (Anti-CD62P antibody [Psel.KO.2.7]) Click here (or use the following: https://www.abcam.com/index.html?datasheet=54427). Unfortunately, we do not have any antibodies against CD45, CD11b or ETAR (endothelin type A receptor in horse available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html ETAR from horse shows a 96% homology with human and you might be eligible for our testing discount program. Please let me know what application are planned with this antibody. I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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