Anti-CD62P antibody [Psel.KO.2.7] (ab54427)
![Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)](https://www.abcam.com/ps/products/54/ab54427/Images/ab54427-367827-anticd62ppselko27ihcfrhutonsil.jpg "Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)")
Key features and details
- Mouse monoclonal [Psel.KO.2.7] to CD62P
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD62P antibody [Psel.KO.2.7]
See all CD62P primary antibodies -
Description
Mouse monoclonal [Psel.KO.2.7] to CD62P -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep
-
Immunogen
Tissue, cells or virus corresponding to Human CD62P.
-
Positive control
- Flow Cyt: Human whole blood; IHC-Fr: Human tonsil tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
Psel.KO.2.7 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab54427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 5 µg/ml. | |
| Flow Cyt | Use a concentration of 0.2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
Tissue specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
Involvement in disease
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6403 Human
- Omim: 173610 Human
- SwissProt: P16109 Human
- Unigene: 73800 Human
-
Alternative names
- Antigen CD62 antibody
- CD62 antibody
- CD62 antigen-like family member P antibody
see all
Images
-
Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)
IHC image of CD62P staining in a section of frozen normal human tonsil*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab54427 at 5µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
![Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)](https://www.abcam.com/ps/products/54/ab54427/Images/ab54427-412843-anti-cd62p-antibody-psel-ko-2-7-flow-cytometry-whole-blood-cells-human.jpg)
Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.7] (ab54427)Flow cytometry staining of human whole blood with ab54427 (right) or mouse IgG1κ (ab170190) isotype (left). Red blood cells of 200 µL blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab54427) or mouse IgG1κ (ab170190) isotype (1x106 in 100 µL at 0.2 µg/mL) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD42a PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on alive neutrophils.
Protocols
Datasheets and documents
References (6)
ab54427 has been referenced in 6 publications.
- Shen DS et al. Aspirin eugenol ester inhibits agonist-induced platelet aggregation in vitro by regulating PI3K/Akt, MAPK and Sirt 1/CD40L pathways. Eur J Pharmacol 852:1-13 (2019). PubMed: 30797789
- Su C et al. Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation. Front Pharmacol 10:990 (2019). PubMed: 31619988
- Li XH et al. Effect of Platelet-derived P-selectin on Neutrophil Recruitment in a Mouse Model of Sepsis-induced Acute Kidney Injury. Chin Med J (Engl) 130:1694-1699 (2017). Mouse . PubMed: 28685720
- Holloway PM et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
- Baron DM et al. Adverse effects of hemorrhagic shock resuscitation with stored blood are ameliorated by inhaled nitric oxide in lambs*. Crit Care Med 41:2492-501 (2013). Sheep . PubMed: 23887236
- Massaguer A et al. Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue Antigens 56:117-28 (2000). IHC-Fr, Flow Cyt ; Mouse, Rat, Human . PubMed: 11019911
