Question (49657) | Anti-CD62P antibody [Psel.KO.2.7] (ab54427)

Go to datasheet (ab54427)

Question

We have purchased some antibodies against rat platelets:

Primary:

ab108949 - mouse monoclonal anti CD63, FITC labeled
(ab106163 as isotype control)

ab54427 - mouse monoclonal anti CD62P
(ab91353 as the isotype control, as a secondary antibody)

ab95780 anti CD42d armenian hamster antibody
(ab18479 as an isotype control, for which we are using APC-conjugated Goat Anti-Hamster IgG from Leinco).

We are ibodies.doing flow cytometry experiments on fixed whole blood, and fixed platelet-rich plasma. The problem is that the samples with the isotype control show the same fluorescence as the samples with the primary ant We have tried a number of different fixing and staining protocols, with and without washing, but until now had no success. What I am wondering about is, how reliable are these primary antibodies for the use with rat platelets?

Some help would be greatly appreciated.

Answer

Thank you for your reply.

I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

I look forward to your reply and helping resolve this matter.