Anti-CD63 antibody (ab216130)

Rabbit polyclonal CD63 antibody. Validated in WB, IHC and tested in Mouse, Human. Cited in 1 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to CD63
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CD63. The exact sequence is proprietary. NP_001244318
    Database link: P08962

  • Positive control

    • WB: NIH/3T3, K562 and HEK-293T cell lysates. IHC-P: Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab216130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/200 - 1/2000. Predicted molecular weight: 26 kDa.

Target

  • Function

    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificity

    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Lysosome-associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Images

  • All lanes : Anti-CD63 antibody (ab216130) at 1/200 dilution

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 40 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 26 kDa


    Exposure time: 20 seconds


    12% SDS-PAGE

  • Paraffin-embedded human liver cancer tissue stained for CD63 with ab216130 at a 1/25 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Wang P  et al. Exosomes from M1-Polarized Macrophages Enhance Paclitaxel Antitumor Activity by Activating Macrophages-Mediated Inflammation. Theranostics 9:1714-1727 (2019). Read more (PubMed: 31037133) »
  • Liu Y  et al. Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis. J Physiol Biochem 75:391-401 (2019). Read more (PubMed: 31321740) »
See all 5 Publications for this product

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