Product nameAnti-CD63 antibody
See all CD63 primary antibodies
DescriptionRabbit polyclonal to CD63
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human CD63. The exact sequence is proprietary. NP_001244318
Database link: P08962
- WB: NIH/3T3, K562 and HEK-293T cell lysates. IHC-P: Human liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab216130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/200 - 1/2000. Predicted molecular weight: 26 kDa.|
FunctionThis antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
Tissue specificityDysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
Cellular localizationCell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
- Information by UniProt
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
All lanes : Anti-CD63 antibody (ab216130) at 1/200 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 20 seconds
Paraffin-embedded human liver cancer tissue stained for CD63 with ab216130 at a 1/25 dilution in immunohistochemical analysis.
This product has been referenced in:
- Wang P et al. Exosomes from M1-Polarized Macrophages Enhance Paclitaxel Antitumor Activity by Activating Macrophages-Mediated Inflammation. Theranostics 9:1714-1727 (2019). Read more (PubMed: 31037133) »
- Liu Y et al. Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis. J Physiol Biochem 75:391-401 (2019). Read more (PubMed: 31321740) »