Recombinant Anti-CD63 antibody [EPR21151] (ab217345)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-CD63 antibody [EPR21151]
See all CD63 primary antibodies -
Description
Rabbit monoclonal [EPR21151] to CD63 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse CD63 aa 100-200. The exact sequence is proprietary.
Database link: P41731 -
Positive control
- WB: bEND.3, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse kidney lysate. ICC/IF: NIH/3T3 and bEND.3 cells.
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General notes
Human and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21151 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab217345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa). | |
| ICC/IF | 1/100. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 12512 Mouse
- SwissProt: P41731 Mouse
- Unigene: 371552 Mouse
- Unigene: 416079 Mouse
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291242-anti-cd63-antibody-epr21151-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291241-anti-cd63-antibody-epr21151-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Western blot - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291243-anti-cd63-antibody-epr21151-western-blot.jpg)
Western blot - Anti-CD63 antibody [EPR21151] (ab217345)Lanes 1-3 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/2000 dilution
Lane 4 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/1000 dilution
Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-3: 3 minutes; Lane 4: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular masses observed are consistent with what has been described in the literature (PMID: 9890706; PMID: 9790070).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Datasheets and documents
References
This product has been referenced in:
- Pua HH et al. Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses. Cell Rep 26:933-944.e4 (2019). Read more (PubMed: 30673615) »
- Song L et al. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a. Nat Commun 10:1639 (2019). Read more (PubMed: 30967557) »
