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RecombinantRabMAb

Recombinant Anti-CD63 antibody [EPR21151] (ab217345)

  • Datasheet
  • Certificate of Compliance
Reviews (3) Submit a question References (19)

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Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)
  • Western blot - Anti-CD63 antibody [EPR21151] (ab217345)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)
  • Anti-CD63 antibody [EPR21151] (ab217345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21151] to CD63
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-CD63 antibody [EPR21151]
    See all CD63 primary antibodies
  • Description

    Rabbit monoclonal [EPR21151] to CD63
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse CD63 aa 100-200. The exact sequence is proprietary.
    Database link: P41731

  • Positive control

    • WB: bEND.3, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse kidney lysate. ICC/IF: NIH/3T3 and bEND.3 cells.
  • General notes

    Human and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21151
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)
    • Alexa Fluor® 488 Anti-CD63 antibody [EPR21151] (ab233055)
    • Alexa Fluor® 647 Anti-CD63 antibody [EPR21151] (ab233056)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

Our Abpromise guarantee covers the use of ab217345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa).
ICC/IF 1/100.

Target

  • Function

    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificity

    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Target information above from: UniProt accession P08962 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12512 Mouse
    • SwissProt: P41731 Mouse
    • Unigene: 371552 Mouse
    • Unigene: 416079 Mouse
    • Alternative names

      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Lysosome-associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)
      Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)

      Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-CD63 antibody [EPR21151] (ab217345)
      Western blot - Anti-CD63 antibody [EPR21151] (ab217345)
      Lanes 1-3 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/2000 dilution
      Lane 4 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/1000 dilution

      Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg
      Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
      Lane 3 : Mouse kidney lysate at 20 µg
      Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 40-60 kDa
      why is the actual band size different from the predicted?



       Exposure times: Lanes 1-3: 3 minutes; Lane 4: 5 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular masses observed are consistent with what has been described in the literature (PMID: 9890706; PMID: 9790070).

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)
      Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)

      Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Anti-CD63 antibody [EPR21151] (ab217345)
      Anti-CD63 antibody [EPR21151] (ab217345)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (19)

    Publishing research using ab217345? Please let us know so that we can cite the reference in this datasheet.

    ab217345 has been referenced in 19 publications.

    • Liang Y  et al. Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. J Extracell Vesicles 9:1697583 (2020). PubMed: 31853339
    • Tan CF  et al. Exploring Extracellular Vesicles Biogenesis in Hypothalamic Cells through a Heavy Isotope Pulse/Trace Proteomic Approach. Cells 9:N/A (2020). PubMed: 32466345
    • Liu H  et al. Colorectal cancer-derived exosomal miR-106b-3p promotes metastasis by down-regulating DLC-1 expression. Clin Sci (Lond) 134:419-434 (2020). PubMed: 32065214
    • Qiu L  et al. Exosomal microRNA-146a derived from mesenchymal stem cells increases the sensitivity of ovarian cancer cells to docetaxel and taxane via a LAMC2-mediated PI3K/Akt axis. Int J Mol Med 46:609-620 (2020). PubMed: 32626953
    • Wang D  et al. Elevated plasma levels of exosomal BACE1-AS combined with the volume and thickness of the right entorhinal cortex may serve as a biomarker for the detection of Alzheimer's disease. Mol Med Rep 22:227-238 (2020). PubMed: 32377715
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD63 antibody [EPR21151]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (OC-2 cell (organ of Corti))
    Permeabilization
    Yes - 0.3% Triton x100
    Specification
    OC-2 cell (organ of Corti)
    Blocking step
    BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2020

    Immunohistochemistry free floating abreview for Anti-CD63 antibody [EPR21151]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain (Fixed with PFA))
    Specification
    Brain (Fixed with PFA)
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD63 antibody [EPR21151]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bone marrow derived mast cells)
    Permeabilization
    Yes - 0.1% Triton-X 100
    Specification
    Bone marrow derived mast cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Nicole Corder

    Verified customer

    Submitted Jul 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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