Recombinant Anti-CD63 antibody [EPR5702] (ab134045)
![Western blot - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-317986-anti-cd63-antibody-epr5702-western-blot.jpg "Western blot - Anti-CD63 antibody [EPR5702] (ab134045)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5702] to CD63
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD63 antibody [EPR5702]
See all CD63 primary antibodies -
Description
Rabbit monoclonal [EPR5702] to CD63 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD63 aa 100-200 (extracellular). The exact sequence is proprietary.
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Positive control
- WB: Human platelet, HL-60 and A375 cell lysates and human melanoma tissue lysate. Brefeldin A treated wild-type HAP1 whole cell lysate. IHC-P: Human melanoma and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5702 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 30-65 kDa (predicted molecular weight: 26 kDa).
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| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 30-65 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Western blot - Anti-CD63 antibody [EPR5702] (ab134045)All lanes : Anti-CD63 antibody [EPR5702] (ab134045) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Brefeldin A treated wild-type HAP1 whole cell lysate
Lane 3 : CD63 knockout HAP1 whole cell lysate
Lane 4 : Brefeldin A treated CD63 knockout HAP1 whole cell lysate
Lane 5 : HL60 whole cell lysate
Lane 6 : Human platelets whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 26 kDaLanes 1 - 6: Merged signal (red and green). Green - ab134045 observed at 26 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab134045 was shown to specifically react with CD63 in wild-type HAP1 Brefeldin A treated cells as signal was lost in HAP1 Brefeldin A treated CD63 knockout cells. Wild-type and CD63 knockout samples were subjected to SDS-PAGE. Ab134045 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-253881-anti-cd63-antibody-epr5702-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD63 with purified ab134045 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-253920-anti-cd63-antibody-epr5702-western-blot.jpg)
Western blot - Anti-CD63 antibody [EPR5702] (ab134045)All lanes : Anti-CD63 antibody [EPR5702] (ab134045) at 1/5000 dilution (purified)
Lane 1 : A375 whole cell lysate
Lane 2 : HL-60 whole cell lysate
Lane 3 : Human melanoma tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 30-65 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-148141-anti-cd63-antibody-epr5702-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling CD63 with unpurified ab134045 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-253882-anti-cd63-antibody-epr5702-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] (ab134045)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue (negative control) labelling CD63 with purified ab134045 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-148142-anti-cd63-antibody-epr5702-western-blot.jpg)
Western blot - Anti-CD63 antibody [EPR5702] (ab134045)All lanes : Anti-CD63 antibody [EPR5702] (ab134045) at 1/1000 dilution (unpurified)
Lane 1 : Human platelet lysate
Lane 2 : Human melanoma lysate
Lane 3 : HL-60 lysate
Lane 4 : A375 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 26 kDa -
![Anti-CD63 antibody [EPR5702] (ab134045)](https://www.abcam.com/ps/products/134/ab134045/Images/ab134045-8-benefits-of-recombinant-antibodies.png)
Anti-CD63 antibody [EPR5702] (ab134045)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (94)
ab134045 has been referenced in 94 publications.
- Wei D et al. RAB31 marks and controls an ESCRT-independent exosome pathway. Cell Res 31:157-177 (2021). PubMed: 32958903
- Ma K et al. Articular chondrocyte-derived extracellular vesicles promote cartilage differentiation of human umbilical cord mesenchymal stem cells by activation of autophagy. J Nanobiotechnology 18:163 (2020). PubMed: 33167997
- Chen QL et al. microRNAs carried by exosomes promote epithelial-mesenchymal transition and metastasis of liver cancer cells. Am J Transl Res 12:6811-6826 (2020). PubMed: 33194074
- Su X et al. FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner. Cell Death Discov 6:140 (2020). PubMed: 33293521
- Huang S et al. microRNA-148a-3p in extracellular vesicles derived from bone marrow mesenchymal stem cells suppresses SMURF1 to prevent osteonecrosis of femoral head. J Cell Mol Med 24:11512-11523 (2020). PubMed: 32871042