Recombinant Anti-CD63 antibody [KILL150A] (ab271286)
![Western blot - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-395330-anticd63-antibody-kill150a-western-blot-skmel28.jpg "Western blot - Anti-CD63 antibody [KILL150A] (ab271286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KILL150A] to CD63
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD63 antibody [KILL150A]
See all CD63 primary antibodies -
Description
Mouse monoclonal [KILL150A] to CD63 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-MEL-28 and HUVEC whole cell lysate. Human lymph node tissue lysate. IHC-P: Human melanoma tissue. ICC/IF: SK-MEL-28 cells. Flow Cyt: SK-MEL-28
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KILL150A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 0.978 µg/ml. Predicted molecular weight: 26 kDa.
|
| IHC-P | (1) |
Use a concentration of 0.978 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Flow Cyt |
Use a concentration of 19.56 µg/ml.
|
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| ICC/IF |
Use a concentration of 19.56 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.978 µg/ml. Predicted molecular weight: 26 kDa. |
|
IHC-P
Use a concentration of 0.978 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use a concentration of 19.56 µg/ml. |
|
ICC/IF
Use a concentration of 19.56 µg/ml. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Western blot - Anti-CD63 antibody [KILL150A] (ab271286)All lanes : Anti-CD63 antibody [KILL150A] (ab271286) at 0.978 µg/ml
Lane 1 : SK-MEL-28 (human malignant melanoma), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : Human lymph node tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaCD63 can undergo glycosylation as shown in lane 1, 2 and 3 (PMID: 9890706, 28740179).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 3 mins; Lane 3: 48 secs.
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-1-anticd63-antibody-kill150a-immunohistochemistry-human-melanoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human melanoma. The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-2-anticd63-antibody-kill150a-immunohistochemistry-human-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [KILL150A] (ab271286)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD63 with ab271286 at 1/4000 dilution (0.978µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab271268 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: Nearly no staining on human breast.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-3-anticd63-antibody-kill150a-immunocytochemistry-skmel28.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [KILL150A] (ab271286)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling CD63 with ab271286 at 1/50 dilution (19.56µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing lysosome/endosomes (ab252919) co-staining in SK-MEL-28 cells.
ab252919 Anti-CD63 rabbit monoclonal antibody was used to counterstain tubulin at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab271286 at a 1/50 dilution (19.56µg/ml) followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab259919 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry - Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-5-anticd63-antibody-kill150a-flow-cytometry-skmel28.jpg)
Flow Cytometry - Anti-CD63 antibody [KILL150A] (ab271286)Flow cytometric analysis of SK-MEL-28 (human malignant melanoma) cells labelling CD63 with ab271286 at 1/1000 dilution (19.56µg/ml) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-CD63 antibody [KILL150A] (ab271286)](https://www.abcam.com/ps/products/271/ab271286/Images/ab271286-7-benefits-of-recombinant-antibodies.png)
Anti-CD63 antibody [KILL150A] (ab271286)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab271286 has been referenced in 1 publication.
- Li J et al. Hypoxic colorectal cancer-derived extracellular vesicles deliver microRNA-361-3p to facilitate cell proliferation by targeting TRAF3 via the noncanonical NF-?B pathways. Clin Transl Med 11:e349 (2021). PubMed: 33784010
