• Product name

    Anti-CD63 antibody [MEM-259]
    See all CD63 primary antibodies
  • Description

    Mouse monoclonal [MEM-259] to CD63
  • Host species

  • Specificity

    CD63 antigen (human)
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). (HPB-ALL T cell line)

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.



Our Abpromise guarantee covers the use of ab8219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml.

Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity

      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Information by UniProt
    • Database links

    • Alternative names

      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Lysosome-associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all


    • ab8219 staining CD63 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody which was used diluted to 10 µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

      See Abreview

    • ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

      See Abreview

    • IHC image of CD63 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8219, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml). 


    This product has been referenced in:

    • García-Romero N  et al. Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation. J Transl Med 17:75 (2019). Read more (PubMed: 30871557) »
    • Ortega FG  et al. EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform. Talanta 194:243-252 (2019). Read more (PubMed: 30609526) »
    See all 53 Publications for this product

    Customer reviews and Q&As

    1-10 of 17 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Human Cell (Blood film)
    Blood film
    Yes - 0.1M PBS with 1% TritonX100

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 26 2014


    ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

    The gel should be run in non-reducing conditions as these antibodies do not recognise their antigens well when the western blot sample contains reducing agent. We therefore advise against adding beta-mercaptoethanol or DTT in the loading buffer.

    Read More


    We have not tested the stability of ab8219 under the alkaline conditions that you described; thus, we cannot guarantee that it will work under these conditions. The laboratory thinks it is possible that it could work, but only performing the experiment will tell.

    Read More


    We are not aware of a reference describing production and characterization of this CD63 antibody. The epitope the antibody recognizes on CD63 has not, to our knowledge, been mapped, but we do know that the epitope is extracellular when CD63 is integrated into the plasma membrane.

    Read More


    Thank you for contacting us.

    The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

    Please do not hesitate to contact us if you need any more advice or information.

    Read More


    Thank you for your inquiry.

    Ab8219 (anti-CD63) is supplied as 100ul at a concentration of 1mg/ml. We have a customer who reported successfully using the antibody at a 1/200 dilution (0.2 mg/ml), but it may be necessary to optimize this for your own experiments. Please note that we recommend using non-reducing conditions for the western blot (i.e. no BME or DTT).

    I hope this information helps. Please do not hesitate to contact us if you need anything further. Have a wonderful day, and best of luck in your research!

    Read More


    Thank you for your enquiry.

    I can confirm that the epitope for ab8219 CD63 antibody [MEM-259] has been mapped to the extracellular region of the CD63 protein.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More


    Thnak you for contacting Abcam.

    In regards to ab8219, I have had the chance to talk with the lab. According to them this antigen is indeed very variable on WB of various material. I am sending the pattern they obtained on non-reducing K562 cells (see image CCE3541736_MEM-259WB.jpg), however observing more heavier bands using other material would be also a reasonable result.

    I have attached information given to me by the lab producing ab59479 (see remainng attached). These include a puplication which has used the product in Western blot as well as a Powerpoint file which contains lab testing results.

    I hope this information is helpful. Please do not hestitate to contact me if you have any questions.

    Read More


    Thank you for contacting us.

    CD63 antigen is also known as LIMP and LAMP-3. While unmodified protein is about 25kDA, this is heavily glycolylated. AB59476migrates like a smear due to a very complexe N-glycosylation. I would recommend this product because this real world information is available whereas the data for ab8219 appears to be based on theory and not practise.

    Please remember that differences exist between the cell lines (e.g. K562 givesbetter migration than HEL).

    It is important to remember that this antibody (as well that all theanti-tetraspanine antibodies) must be used under non-reduced conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More


    The antibody was validated for western blotting using these conditions:

    Sample: K562 cells (24 millions, lysed with 1% laurylmaltoside in 700 ul), non-reducing SDS, denatured by heat for 5 min.
    Blocking: 5% milk, 4oC overnight
    Primary antibody: diluted 1:100 in blocking solution, room temperature, 60 min.

    I hope this is helpful. If you have any questions, please do not hesitate to contact us.

    Read More

    1-10 of 17 Abreviews or Q&A

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