Anti-CD63 antibody [NK1/C3] (ab1318)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.com/ps/products/1/ab1318/Images/ab1318-4-anti-cd63-antibody-nk1-c3-immunohistochemistry-melanoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)")
Key features and details
- Mouse monoclonal [NK1/C3] to CD63
- Suitable for: ICC/IF, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD63 antibody [NK1/C3]
See all CD63 primary antibodies -
Description
Mouse monoclonal [NK1/C3] to CD63 -
Host species
Mouse -
Specificity
This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells. -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD63. Smooth plasma membrane fraction of MeWo cells.
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Positive control
- ICC/IF: HAP1 cells (HAP1-CD63 knockout cells used as negative cell line)
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General notes
CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Primary antibody notes
CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40. -
Clonality
Monoclonal -
Clone number
NK1/C3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
Signal can be observed in cells fixed with methanol or PFA. |
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| IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt |
1/100.
(Also see PMID 19129916). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. Signal can be observed in cells fixed with methanol or PFA. |
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IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt
1/100. (Also see PMID 19129916). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)Formalin-fixed, paraffin-embeded human melanoma tissue stained for CD63 using ab1318 at 1/100 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.com/ps/products/1/ab1318/Images/ab1318-369936-ICCIFab1318GR3300020101.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [NK1/C3] (ab1318)ab1318 staining CD63 in wild-type HAP1 cells (top panel) and CD63 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab1318 at 1ug/ml and ab6046 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.com/ps/products/1/ab1318/Images/ab1318_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (ab1318)ab1318 staining human melanoma by IHC-P. -
![Flow Cytometry - Anti-CD63 antibody [NK1/C3] (ab1318)](https://www.abcam.com/ps/products/1/ab1318/Images/CD63-Primary-antibodies-ab1318-3.jpg)
Flow Cytometry - Anti-CD63 antibody [NK1/C3] (ab1318)Overlay histogram showing HL60 cells stained with ab1318 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1318, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab1318 has been referenced in 15 publications.
- Ivanusic D et al. The large extracellular loop of CD63 interacts with gp41 of HIV-1 and is essential for establishing the virological synapse. Sci Rep 11:10011 (2021). PubMed: 33976357
- Oh JG et al. Analysis of extracellular vesicle miRNA profiles in heart failure. J Cell Mol Med 24:7214-7227 (2020). PubMed: 32485073
- Albacete-Albacete L et al. ECM deposition is driven by caveolin-1-dependent regulation of exosomal biogenesis and cargo sorting. J Cell Biol 219:N/A (2020). PubMed: 33053168
- Yokoi A et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
- Aaberg-Jessen C et al. Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas. BMC Cancer 18:270 (2018). PubMed: 29523123
