Anti-CD63 antibody [NKI/C3] - BSA and Azide free (ab213090)

Overview

  • Product name

    Anti-CD63 antibody [NKI/C3] - BSA and Azide free
    See all CD63 primary antibodies
  • Description

    Mouse monoclonal [NKI/C3] to CD63 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus. Smooth plasma membrane fraction of MeWo cells
    Database link: P08962

  • Positive control

    • Flow Cytometry: MCF7 cells. IHC-P: Mouse spleen tissue. Human melanoma tissue. WB: HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab213090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificity

    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Lysosome-associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Images

  • Anti-CD63 antibody [NKI/C3] - BSA and Azide free (ab213090) at 1 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
  • Immunohistochemical analysis of paraffin embedded, formalin fixed mouse spleen tissue labeling CD63 with ab213090 at 1 μg/mL dilution. DAB detection.

  • Immunohistochemical analysis of paraffin embedded, formalin fixed human melanoma tissue labeling CD63 with ab213090 at 1 μg/mL dilution. EAC chromogen labeling.

  • Flow cytometry analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling CD63 using PE conjugated ab213090 (Red). Isotype control in Green; cells alone in black.

References

This product has been referenced in:

  • Zhao P  et al. Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7. Biochim Biophys Acta Gen Subj 1863:502-510 (2019). Read more (PubMed: 30528490) »
  • Li Y  et al. Characterizing the landscape of peritoneal exosomal microRNAs in patients with ovarian cancer by high-throughput sequencing. Oncol Lett 17:539-547 (2019). Read more (PubMed: 30655799) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow derived mast cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Bone marrow derived mast cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Nicole Corder

Verified customer

Submitted Aug 07 2018

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