Immunocytochemistry/ Immunofluorescence abreview for Anti-CD63 antibody [NKI/C3] - BSA and Azide free

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow derived mast cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Bone marrow derived mast cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in 1x PBS
Concentration
1 µg/ml

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Thermo FIsher donkey anti-mouse Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
No signal was detected, even when using the recommended concentration of antibody.
Abcam response
We have been in contact with the researcher and resolved the issue in accordance with our Abpromise.

Mrs. Nicole Corder

Verified customer

Submitted Aug 07 2018

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