Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)
![Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)](https://www.abcam.com/ps/products/59/ab59479/Images/CD63-Primary-antibodies-ab59479-2.jpg "Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)")
Key features and details
- Mouse monoclonal [TS63] to CD63 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD63 antibody [TS63] - BSA and Azide free
See all CD63 primary antibodies -
Description
Mouse monoclonal [TS63] to CD63 - BSA and Azide free -
Host species
Mouse -
Specificity
Recognises the LIMP antigen, a 30-60 kda (smear) protein. -
Tested applications
Suitable for: ICC, Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD63. Jurkat and HEL cell lines.
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Positive control
- ICC: A431 cells; IHC-P: Human Skin Melanoma; Flow Cyt: HL60 cells; WB: HEL (human erythroleukemia cell line) cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Sterile-filtered through 0.22 µm and treated to remove endotoxins. -
Clonality
Monoclonal -
Clone number
TS63 -
Myeloma
P3-X63 Ag8.3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
|
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (1) |
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa).
|
| Notes |
|---|
|
ICC
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)
ICC image of ab59479 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59479 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [TS63] (ab59479)](https://www.abcam.com/ps/products/59/ab59479/Images/ab59479-210431-ab59479GR589165Edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [TS63] (ab59479)IHC image of CD63 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59479, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Anti-CD63 antibody [TS63] (ab59479)](https://www.abcam.com/ps/products/59/ab59479/Images/CD63-Primary-antibodies-ab59479-3.jpg)
Flow Cytometry - Anti-CD63 antibody [TS63] (ab59479)Overlay histogram showing HL60 cells stained with ab59479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59479, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight®488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (167)
ab59479 has been referenced in 167 publications.
- Ke Y et al. Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90. Stem Cell Res Ther 12:21 (2021). PubMed: 33413616
- Wu H et al. miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis. J Cell Mol Med 25:1896-1910 (2021). PubMed: 33465281
- Shan G et al. Downregulated exosomal microRNA-148b-3p in cancer associated fibroblasts enhance chemosensitivity of bladder cancer cells by downregulating the Wnt/ß-catenin pathway and upregulating PTEN. Cell Oncol (Dordr) 44:45-59 (2021). PubMed: 33423167
- Kuang MJ et al. Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway. Mol Ther Nucleic Acids 23:565-576 (2021). PubMed: 33510944
- Zhang P et al. Distinct mRNAs in Cancer Extracellular Vesicles Activate Angiogenesis and Alter Transcriptome of Vascular Endothelial Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33921957