Anti-CD64 antibody (ab203349)
Key features and details
- Rabbit polyclonal to CD64
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CD64 antibody
See all CD64 primary antibodies -
Description
Rabbit polyclonal to CD64 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human CD64 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P12314 -
Positive control
- Rat brain and lung tissues; Human colon carcinoma lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
(or 1/50 - 1/200 using a fluorescent secondary antibody). |
|
WB |
1/100 - 1/1000. Predicted molecular weight: 43 kDa.
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Notes |
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IHC-P
1/100 - 1/500. (or 1/50 - 1/200 using a fluorescent secondary antibody). |
WB
1/100 - 1/1000. Predicted molecular weight: 43 kDa. |
Target
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Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
Tissue specificity
Monocyte/macrophage specific. -
Sequence similarities
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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Database links
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Entrez Gene: 14129 Mouse
- Entrez Gene: 295279 Rat
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
see all -
Alternative names
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
Images
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All lanes : Anti-CD64 antibody (ab203349) at 1/200 dilution
Lane 1 : Rat lung lysate
Lane 2 : Human colon carcinoma lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 43 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling CD64 with ab203349 at 1/200 dilution, followed by conjugation to the secondary antibody.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling CD64 with ab203349 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203349 has not yet been referenced specifically in any publications.