Product nameAnti-CD64 antibody [EPR4623]
See all CD64 primary antibodies
DescriptionRabbit monoclonal [EPR4623] to CD64
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
corresponding to Human CD64 aa 50-150.
- U937, THP1, and HL60 cell lysates; U937 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHigh affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Tissue specificityMonocyte/macrophage specific.
Sequence similaritiesBelongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated on serine residues.
Cellular localizationCell membrane. Stabilized at the cell membrane through interaction with FCER1G.
- Information by UniProt
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 64-78 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zhang H et al. Fc?RI (CD64) contributes to the severity of immune inflammation through regulating NF-?B/NLRP3 inflammasome pathway. Life Sci 207:296-303 (2018). Read more (PubMed: 29920250) »
- Lin HT et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev 2:15046 (2015). Read more (PubMed: 26682238) »