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Rabbit monoclonal [EPR4623] to CD64
, Flow Cytmore detailsUnsuitable for:
corresponding to Human CD64 aa 50-150.
- U937, THP1, and HL60 cell lysates; U937 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
||1/10 - 1/100.
||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P.
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Phosphorylated on serine residues.
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
Information by UniProt
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
Western blot - Anti-CD64 antibody [EPR4623] (ab109449)
All lanes :
Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilutionLane 1 :
U937 cell lysate Lane 2 :
THP1 cell lysateLane 3 :
HL60 cell lysate
Lysates/proteins at 10 µg per lane.Predicted band size:
43 kDaObserved band size:
64-78 kDa (why is the actual band size different from the predicted?
Flow Cytometry - Anti-CD64 antibody [EPR4623] (ab109449)
ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
Other - Anti-CD64 antibody [EPR4623] (ab109449)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"