Recombinant
RabMAb

Anti-CD64 antibody [EPR4623] (ab109449)

Overview

  • Product name
    Anti-CD64 antibody [EPR4623]
    See all CD64 primary antibodies
  • Description
    Rabbit monoclonal [EPR4623] to CD64
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human CD64 aa 50-150.

  • Positive control
    • U937, THP1, and HL60 cell lysates; U937 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.00 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4623
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
    • Tissue specificity
      Monocyte/macrophage specific.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. FCGR1 family.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localization
      Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 64 antibody
      • CD64 antibody
      • CD64 antigen antibody
      • CD64A antibody
      • CD64b antibody
      • CD64c antibody
      • Fc fragment of IgG high affinity Ia receptor antibody
      • Fc fragment of IgG high affinity Ib receptor antibody
      • Fc fragment of IgG high affinity Ic receptor antibody
      • Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
      • Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody
      • Fc fragment of IgG, high affinity Ia, receptor for antibody
      • Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody
      • Fc fragment of IgG, high affinity Ib, receptor for antibody
      • Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody
      • Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody
      • Fc fragment of IgG, high affinity Ic, receptor for antibody
      • Fc gamma receptor antibody
      • Fc gamma receptor I A1 antibody
      • Fc gamma receptor I antibody
      • Fc gamma receptor I B2 antibody
      • Fc gamma RI antibody
      • Fc gamma RIA antibody
      • Fc gamma RIB antibody
      • Fc of IgG high affinity Ia receptor antibody
      • Fc-gamma RI antibody
      • Fc-gamma RIA antibody
      • Fc-gamma RIC antibody
      • FCG 1 antibody
      • FCG1 antibody
      • FcgammaRI antibody
      • FcgammaRIa antibody
      • FCGR 1 antibody
      • FCGR1 antibody
      • FCGR1_HUMAN antibody
      • FCGR1A antibody
      • FCGR1B antibody
      • FCGR1C antibody
      • FcRI antibody
      • FcRIB antibody
      • FCRIC antibody
      • FLJ18345 antibody
      • HFcgammaRIB antibody
      • High affinity immunoglobulin gamma Fc receptor I antibody
      • High affinity immunoglobulin gamma Fc receptor IB antibody
      • IGFR 1 antibody
      • IGFR1 antibody
      • IGFRB antibody
      • IGFRC antibody
      • IgG Fc receptor I antibody
      • IgG Fc receptor IB antibody
      • IgG Fc receptor IC antibody
      • Immunoglobulin G Fc receptor I antibody
      • Immunoglobulin G Fc receptor IB antibody
      • Immunoglobulin G Fc receptor IC antibody
      • MGC137713 antibody
      see all

    Images

    • All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : THP1 cell lysate
      Lane 3 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa
      Observed band size: 64-78 kDa (why is the actual band size different from the predicted?)

    • ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab109449 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109449.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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