Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4623] to CD64
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-CD64 antibody [EPR4623]
See all CD64 primary antibodies
DescriptionRabbit monoclonal [EPR4623] to CD64
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
corresponding to Human CD64 aa 50-150.
- U937, THP1, and HL60 cell lysates; U937 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHigh affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Tissue specificityMonocyte/macrophage specific.
Sequence similaritiesBelongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated on serine residues.
Cellular localizationCell membrane. Stabilized at the cell membrane through interaction with FCER1G.
- Information by UniProt
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
ab109449 at 1/100 dilution staining CD64 in U937 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
All lanes : Anti-CD64 antibody [EPR4623] (ab109449) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 64-78 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab109449 has been referenced in 2 publications.
- Zhang H et al. Fc?RI (CD64) contributes to the severity of immune inflammation through regulating NF-?B/NLRP3 inflammasome pathway. Life Sci 207:296-303 (2018). PubMed: 29920250
- Lin HT et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev 2:15046 (2015). PubMed: 26682238