Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4624] to CD64 - BSA and Azide free
- Suitable for: WB, IP, ICC
- Reacts with: Human
Product nameAnti-CD64 antibody [EPR4624] - BSA and Azide free
See all CD64 primary antibodies
DescriptionRabbit monoclonal [EPR4624] to CD64 - BSA and Azide free
Tested applicationsSuitable for: WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
aa 300-400 (C terminal).
- THP1, HL60, and U937 cell lysates.
ab216857 is the carrier-free version of ab134073 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab216857 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
FunctionHigh affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Tissue specificityMonocyte/macrophage specific.
Sequence similaritiesBelongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated on serine residues.
Cellular localizationCell membrane. Stabilized at the cell membrane through interaction with FCER1G.
- Information by UniProt
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
All lanes : Anti-CD64 antibody [EPR4624] (ab134073) at 1/10000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
This data was developed using ab134073, the same antibody clone in a different buffer formulation.
ab216857 has not yet been referenced specifically in any publications.