Anti-CD64 antibody [OTI3D3] (ab140779)

Mouse monoclonal CD64 antibody [OTI3D3]. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 4 publication(s). Immunogen corresponding to recombinant full length protein.

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Overview

  • Product name

    Anti-CD64 antibody [OTI3D3]
    See all CD64 primary antibodies

  • Description

    Mouse monoclonal [OTI3D3] to CD64

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-374 of Human CD64 produced in HEK293T cell (NP_000557).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human CD64 protein (ab114416) can be used as a positive control in WB. HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA, Human ovary adenocarcinoma tissue, Human lymphoma tissue, COS7 cells transiently transfected by pCMV6-ENTRY CD64

  • General notes

    The clone number has been updated from 3D3 to OTI3D3, both clone numbers name the same clone.

    Dilute in PBS (pH 7.3) prior to use.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, 48% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS

  • Clonality

    Monoclonal

  • Clone number

    OTI3D3

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Predicted molecular weight: 43 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • Function

    High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

  • Tissue specificity

    Monocyte/macrophage specific.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. FCGR1 family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    Phosphorylated on serine residues.

  • Cellular localization

    Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD 64 antibody
    • CD64 antibody
    • CD64 antigen antibody
    • CD64A antibody
    • CD64b antibody
    • CD64c antibody
    • Fc fragment of IgG high affinity Ia receptor antibody
    • Fc fragment of IgG high affinity Ib receptor antibody
    • Fc fragment of IgG high affinity Ic receptor antibody
    • Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ib, receptor for antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody
    • Fc fragment of IgG, high affinity Ic, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma receptor I antibody
    • Fc gamma receptor I B2 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc gamma RIB antibody
    • Fc of IgG high affinity Ia receptor antibody
    • Fc-gamma RI antibody
    • Fc-gamma RIA antibody
    • Fc-gamma RIC antibody
    • FCG 1 antibody
    • FCG1 antibody
    • FcgammaRI antibody
    • FcgammaRIa antibody
    • FCGR 1 antibody
    • FCGR1 antibody
    • FCGR1_HUMAN antibody
    • FCGR1A antibody
    • FCGR1B antibody
    • FCGR1C antibody
    • FcRI antibody
    • FcRIB antibody
    • FCRIC antibody
    • FLJ18345 antibody
    • HFcgammaRIB antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • High affinity immunoglobulin gamma Fc receptor IB antibody
    • IGFR 1 antibody
    • IGFR1 antibody
    • IGFRB antibody
    • IGFRC antibody
    • IgG Fc receptor I antibody
    • IgG Fc receptor IB antibody
    • IgG Fc receptor IC antibody
    • Immunoglobulin G Fc receptor I antibody
    • Immunoglobulin G Fc receptor IB antibody
    • Immunoglobulin G Fc receptor IC antibody
    • MGC137713 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemical analysis of paraffin embedded Human ovary adenocarcinoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
  • Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)
    Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)
    All lanes : Anti-CD64 antibody [OTI3D3] (ab140779) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 43 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD64 using ab140779 at a 1/100 dilution.

References

This product has been referenced in:

  • Zhang L  et al. Glucocorticoids Regulate the Vascular Remodeling of Aortic Dissection Via the p38 MAPK-HSP27 Pathway Mediated by Soluble TNF-RII. EBioMedicine 27:247-257 (2018). Read more (PubMed: 29287621) »
  • Brcic L  et al. Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung. Virchows Arch 472:589-598 (2018). Read more (PubMed: 29520483) »
See all 5 Publications for this product

Publishing research using ab140779? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunocytochemistry abreview for Anti-CD64 antibody [OTI3D3]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Polymorphonuclear Cells)
Permeabilization
No
Specification
Polymorphonuclear Cells
Fixative
Paraformaldehyde
Read More

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Submitted Jul 03 2019

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