Anti-CD64 antibody [OTI3D3] (ab140779)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
  • Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)

Key features and details

  • Mouse monoclonal [OTI3D3] to CD64
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD64 antibody [OTI3D3]
    See all CD64 primary antibodies

  • Description

    Mouse monoclonal [OTI3D3] to CD64

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, African green monkey

  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-374 of Human CD64 produced in HEK293T cell (NP_000557).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human CD64 protein (ab114416) can be used as a positive control in WB. HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA, Human ovary adenocarcinoma tissue, Human lymphoma tissue, COS7 cells transiently transfected by pCMV6-ENTRY CD64

  • General notes

    The clone number has been updated from 3D3 to OTI3D3, both clone numbers name the same clone.

    Dilute in PBS (pH 7.3) prior to use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, 48% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS

  • Clonality

    Monoclonal

  • Clone number

    OTI3D3

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/4000. Predicted molecular weight: 43 kDa.
IHC-P (2)
1/150.
ICC/IF
1/100.
Notes
WB
1/4000. Predicted molecular weight: 43 kDa.
IHC-P
1/150.
ICC/IF
1/100.

Target

  • Function

    High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

  • Tissue specificity

    Monocyte/macrophage specific.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. FCGR1 family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    Phosphorylated on serine residues.

  • Cellular localization

    Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD 64 antibody
    • CD64 antibody
    • CD64 antigen antibody
    • CD64A antibody
    • CD64b antibody
    • CD64c antibody
    • Fc fragment of IgG high affinity Ia receptor antibody
    • Fc fragment of IgG high affinity Ib receptor antibody
    • Fc fragment of IgG high affinity Ic receptor antibody
    • Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ib, receptor for antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody
    • Fc fragment of IgG, high affinity Ic, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma receptor I antibody
    • Fc gamma receptor I B2 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc gamma RIB antibody
    • Fc of IgG high affinity Ia receptor antibody
    • Fc-gamma RI antibody
    • Fc-gamma RIA antibody
    • Fc-gamma RIC antibody
    • FCG 1 antibody
    • FCG1 antibody
    • FcgammaRI antibody
    • FcgammaRIa antibody
    • FCGR 1 antibody
    • FCGR1 antibody
    • FCGR1_HUMAN antibody
    • FCGR1A antibody
    • FCGR1B antibody
    • FCGR1C antibody
    • FcRI antibody
    • FcRIB antibody
    • FCRIC antibody
    • FLJ18345 antibody
    • HFcgammaRIB antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • High affinity immunoglobulin gamma Fc receptor IB antibody
    • IGFR 1 antibody
    • IGFR1 antibody
    • IGFRB antibody
    • IGFRC antibody
    • IgG Fc receptor I antibody
    • IgG Fc receptor IB antibody
    • IgG Fc receptor IC antibody
    • Immunoglobulin G Fc receptor I antibody
    • Immunoglobulin G Fc receptor IB antibody
    • Immunoglobulin G Fc receptor IC antibody
    • MGC137713 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemical analysis of paraffin embedded Human ovary adenocarcinoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
  • Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)
    Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)
    All lanes : Anti-CD64 antibody [OTI3D3] (ab140779) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 43 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)
    Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD64 using ab140779 at a 1/100 dilution.

References (6)

Publishing research using ab140779? Please let us know so that we can cite the reference in this datasheet.

ab140779 has been referenced in 6 publications.

  • Papalampros A  et al. Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPa Expression. Cancers (Basel) 12:N/A (2020). PubMed: 32645996
  • Zhang L  et al. Glucocorticoids Regulate the Vascular Remodeling of Aortic Dissection Via the p38 MAPK-HSP27 Pathway Mediated by Soluble TNF-RII. EBioMedicine 27:247-257 (2018). PubMed: 29287621
  • Brcic L  et al. Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung. Virchows Arch 472:589-598 (2018). PubMed: 29520483
  • Dekker S  et al. Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves. Front Cardiovasc Med 5:105 (2018). PubMed: 30159315
  • Qureshi OS  et al. Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions. Sci Rep 7:17049 (2017). PubMed: 29213127
  • Lara S  et al. Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles. ACS Nano 11:1884-1893 (2017). PubMed: 28112950

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD64 antibody [OTI3D3]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
Permeabilization
Yes - 0.3% Triton X-100
Specification
Colon
Blocking step
10% Donkey Serum + 3% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Dr. Dylan Windell

Verified customer

Submitted Jun 16 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD64 antibody [OTI3D3]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS PH.9
Permeabilization
No
Specification
Lungs
Blocking step
(agent) for 12 minute(s) · Concentration: 0µg/mL · Temperature: RT°C
Fixative
Formaldehyde
Read More

Syeda Mariam Riaz

Verified customer

Submitted Oct 15 2020

Immunocytochemistry abreview for Anti-CD64 antibody [OTI3D3]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Polymorphonuclear Cells)
Permeabilization
No
Specification
Polymorphonuclear Cells
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 03 2019

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