Anti-CD64 antibody [OTI3D3] (ab140779)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156199-anti-cd64-antibody-oti3d3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)")
Key features and details
- Mouse monoclonal [OTI3D3] to CD64
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CD64 antibody [OTI3D3]
See all CD64 primary antibodies -
Description
Mouse monoclonal [OTI3D3] to CD64 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to amino acids 1-374 of Human CD64 produced in HEK293T cell (NP_000557).
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Positive control
- Recombinant Human CD64 protein (ab114416) can be used as a positive control in WB. HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA, Human ovary adenocarcinoma tissue, Human lymphoma tissue, COS7 cells transiently transfected by pCMV6-ENTRY CD64
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General notes
The clone number has been updated from 3D3 to OTI3D3, both clone numbers name the same clone.
Dilute in PBS (pH 7.3) prior to use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI3D3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/4000. Predicted molecular weight: 43 kDa.
|
|
| IHC-P | (1) |
1/150.
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/4000. Predicted molecular weight: 43 kDa. |
|
IHC-P
1/150. |
|
ICC/IF
1/100. |
Target
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Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
Tissue specificity
Monocyte/macrophage specific. -
Sequence similarities
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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Database links
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
Alternative names
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)Immunohistochemical analysis of paraffin embedded Human ovary adenocarcinoma tissue labelling CD64 with ab140779 at a 1/150 dilution.
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![Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156200-anti-cd64-antibody-oti3d3-western-blot.jpg)
Western blot - Anti-CD64 antibody [OTI3D3] (ab140779)All lanes : Anti-CD64 antibody [OTI3D3] (ab140779) at 1/4000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 43 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210076) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156198-anti-cd64-antibody-oti3d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (ab140779)Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling CD64 with ab140779 at a 1/150 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)](https://www.abcam.com/ps/products/140/ab140779/Images/ab140779-156197-anti-cd64-antibody-oti3d3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (ab140779)Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD64 using ab140779 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab140779 has been referenced in 6 publications.
- Papalampros A et al. Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPa Expression. Cancers (Basel) 12:N/A (2020). PubMed: 32645996
- Zhang L et al. Glucocorticoids Regulate the Vascular Remodeling of Aortic Dissection Via the p38 MAPK-HSP27 Pathway Mediated by Soluble TNF-RII. EBioMedicine 27:247-257 (2018). PubMed: 29287621
- Brcic L et al. Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung. Virchows Arch 472:589-598 (2018). PubMed: 29520483
- Dekker S et al. Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves. Front Cardiovasc Med 5:105 (2018). PubMed: 30159315
- Qureshi OS et al. Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions. Sci Rep 7:17049 (2017). PubMed: 29213127
- Lara S et al. Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles. ACS Nano 11:1884-1893 (2017). PubMed: 28112950