Key features and details
- Mouse monoclonal [VIM-2] to CD65s (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Product nameAnti-CD65s antibody [VIM-2] (FITC)
DescriptionMouse monoclonal [VIM-2] to CD65s (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
THP1 (human acute monocytic leukaemia cells).
Clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Primary antibody notesClone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation
Our Abpromise guarantee covers the use of ab74080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
RelevanceCD65s is aberrantly expressed on some acute myeloid leukaemias (AML). During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. CD65s is the sialylated form of human CD65 and is a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.
- CDw65 antibody
ab74080 has been referenced in 1 publication.
- Kniep B et al. The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem 119:456-62 (1996). PubMed: 8830039