Overview

  • Product name

  • Description

    Rabbit polyclonal to CD66b
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total CD66b protein.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD66b (internal sequence).
    Database link: P31997

  • Positive control

    • Human gastric cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The method we used for antigen retrieval is heat induced epitope retrieval, and 10 mM sodium citrate buffer (pH 6) are used for heat-mediated retrieval by a pressure cooker. Once boiling, put the slides to the cooker. As soon as the cooker has reached full pressure, time 4 min.

Target

  • Tissue specificity

    Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carcinoembryonic antigen CGM6 antibody
    • Carcinoembryonic antigen gene family member 6 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 8 antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 8 antibody
    • CD 66b antibody
    • CD 67 antibody
    • CD66b antibody
    • CD66b antigen antibody
    • CD67 antibody
    • CD67 antigen antibody
    • CEACAM 8 antibody
    • CEACAM8 antibody
    • CEAM8_HUMAN antibody
    • CGM 6 antibody
    • CGM6 antibody
    • NCA 95 antibody
    • NCA95 antibody
    • Non-specific cross-reacting antigen NCA-95 antibody
    • Nonspecific cross reacting antigen NCA 95 antibody
    • Nonspecific cross reacting antigen NCA95 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed paraffin embedded human gastric cancer tissue labeling CD66b with ab197678 at a 1/30 dilution.

References

This product has been referenced in:

  • Miksch RC  et al. Prognostic Impact of Tumor-Infiltrating Lymphocytes and Neutrophils on Survival of Patients with Upfront Resection of Pancreatic Cancer. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30609853) »
  • Schoenberg MB  et al. Perivascular Tumor-Infiltrating Leukocyte Scoring for Prognosis of Resected Hepatocellular Carcinoma Patients. Cancers (Basel) 10:N/A (2018). IHC-P ; Human . Read more (PubMed: 30340430) »
See all 3 Publications for this product

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