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    cd66b-antibody-ab214175.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD66b antibody (ab214175)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (ab214175)

    Key features and details

    • Rabbit polyclonal to CD66b
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CD66b antibody
      See all CD66b primary antibodies
    • Description

      Rabbit polyclonal to CD66b
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human CD66b aa 60-110 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      Sequence:

      QDPRGYNWYKGETVDANRRIIGYVISNQQITPGPAYSNRETIYPNASLLM R


      Database link: P31997
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human colon carcinoma tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 1% BSA, 50% Glycerol
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human CD66b protein (ab152024)
    • Related Products

      • Recombinant Human CD66b protein (ab152024)
      • Recombinant Human CD66b protein (ab167763)

    Applications

    Our Abpromise guarantee covers the use of ab214175 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/100 - 1/500.

    Target

    • Tissue specificity

      Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. CEA family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P31997 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1088 Human
      • Omim: 615747 Human
      • SwissProt: P31997 Human
      • Unigene: 41 Human
      • Alternative names

        • Carcinoembryonic antigen CGM6 antibody
        • Carcinoembryonic antigen gene family member 6 antibody
        • Carcinoembryonic antigen related cell adhesion molecule 8 antibody
        • Carcinoembryonic antigen-related cell adhesion molecule 8 antibody
        • CD 66b antibody
        • CD 67 antibody
        • CD66b antibody
        • CD66b antigen antibody
        • CD67 antibody
        • CD67 antigen antibody
        • CEACAM 8 antibody
        • CEACAM8 antibody
        • CEAM8_HUMAN antibody
        • CGM 6 antibody
        • CGM6 antibody
        • NCA 95 antibody
        • NCA95 antibody
        • Non-specific cross-reacting antigen NCA-95 antibody
        • Nonspecific cross reacting antigen NCA 95 antibody
        • Nonspecific cross reacting antigen NCA95 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (ab214175)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (ab214175)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CD66b with ab214175 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab214175? Please let us know so that we can cite the reference in this datasheet.

      ab214175 has been referenced in 1 publication.

      • Zhou C  et al. Development and validation of a seven-immune-feature-based prognostic score for oral squamous cell carcinoma after curative resection. Int J Cancer N/A:N/A (2019). PubMed: 31304591

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab214175.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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