Key features and details
- Mouse monoclonal [GM-2H6] to CD66b
- Suitable for: Flow Cyt, ELISA, Competitive ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD66b antibody [GM-2H6]
See all CD66b primary antibodies
DescriptionMouse monoclonal [GM-2H6] to CD66b
SpecificityThis antibody exclusively recognizes CD66b (CEACAM8).
Tested applicationsSuitable for: Flow Cyt, ELISA, Competitive ELISAmore details
Species reactivityReacts with: Human
The complete extracellular region of human CD66b (CEACAM8) cDNA.
- Flow Cyt: BOSC23 cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
PurityProtein G purified
Purification notesThe antibody was purified from cell culture supernatants.
Our Abpromise guarantee covers the use of ab48589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in leukocytes of chronic myeloid Leukemia patients and bone marrow.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- Carcinoembryonic antigen CGM6 antibody
- Carcinoembryonic antigen gene family member 6 antibody
- Carcinoembryonic antigen related cell adhesion molecule 8 antibody
Ab48589, staining CD66b (CEACAM8) by Flow Cytometry.
BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter was expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, ab48589 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 (CD66b) transfected cells (red curve).
ab48589 has been referenced in 1 publication.
- Yoon J et al. CD66b Regulates Adhesion and Activation of Human Eosinophils. J Immunol 179:8454-62 (2007). Human . PubMed: 18056392