Overview

  • Product name

    Anti-CD68 antibody [3F7D3]
    See all CD68 primary antibodies
  • Description

    Mouse monoclonal [3F7D3] to CD68
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD68 aa 42-155. (Purified from E.coli).
    Sequence:

    VTESTGTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTV HPTSNSTATSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPE PPPPSPSPSPTSKE


    Database link: P34810

  • Positive control

    • Human CD68 (aa42-155) recombinant protein; CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate; HeLa cells; U937, HeLa, HepG2 and Jurkat whole cell lysate (ab7899); Human endometrial cancer and bladder cancer tissues.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR255595-19 are from Tissue Culture Supernatant

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3F7D3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 37 kDa.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.

  • Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution + Human CD68 (aa42-155) recombinant protein

    Predicted band size: 37 kDa



    Expected MWt is 37.4 kDa.

  • All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 37 kDa

  • All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Predicted band size: 37 kDa

  • Immunofluorescent analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Flow cytometric analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.

References

ab201973 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201973.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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