Key features and details
- Rabbit polyclonal to CD68
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD68 antibody
See all CD68 primary antibodies
DescriptionRabbit polyclonal to CD68
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human CD68 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P34810
- Human tonsil tissue; Raji and SH SY5Y cell lysates.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.The user must validate any other storage conditions.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
ab125047 staining CD68 in Mouse vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 5 minutes at 20°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Anti-CD68 antibody (ab125047) at 1/50 dilution + SH SY5Y cell line lysate
Predicted band size: 37 kDa
ab125047, at 1/100, staining CD68 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125047 has been referenced in 12 publications.
- Mori D et al. Pioglitazone strengthen therapeutic effect of adipose-derived regenerative cells against ischemic cardiomyopathy through enhanced expression of adiponectin and modulation of macrophage phenotype. Cardiovasc Diabetol 18:39 (2019). PubMed: 30902059
- Liu L et al. Anti-spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection. JCI Insight 4:N/A (2019). PubMed: 30830861
- Annese T et al. PTX3 Modulates Neovascularization and Immune Inflammatory Infiltrate in a Murine Model of Fibrosarcoma. Int J Mol Sci 20:N/A (2019). PubMed: 31533326
- Guan G et al. Down-regulation of IFIT3 protects liver from ischemia-reperfusion injury. Int Immunopharmacol 60:170-178 (2018). WB ; Mouse . PubMed: 29734133
- Kashiyama N et al. Development of PET Imaging to Visualize Activated Macrophages Accumulated in the Transplanted iPSc-Derived Cardiac Myocytes of Allogeneic Origin for Detecting the Immune Rejection of Allogeneic Cell Transplants in Mice. PLoS One 11:e0165748 (2016). PubMed: 27930666