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    cd68-antibody-ab125212.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD68 antibody (ab125212)

  • Datasheet
  • SDS
Reviews (50)Q&A (1)References (258)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Western blot - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Frozen sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)

Key features and details

  • Rabbit polyclonal to CD68
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CD68 antibody [EPR20545] (ab213363)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CD68 antibody
    See all CD68 primary antibodies
  • Description

    Rabbit polyclonal to CD68
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse CD68 aa 312-326 (internal sequence). Different from the related rat sequence by one amino acid.
    Sequence:

    AFCITRRRQSTYQPL


    Database link: P31996
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Rat and mouse liver tissue, Mouse spleen, skin, and brain tissues; IHC-Fr: Rat liver tissue; WB: Rat and Mouse spleen tissue lysates. Raw264.7 cell lysate.
  • General notes

    View a monoclonal antibody available to this target, ab31630.

    For WB, as CD68 is highly glycosylated, it typically runs between 75-110 kDa depending on the amount of glycosylation in the sample. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.025% Sodium azide
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 5.61% Trehalose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Signal Transduction
    • Antibodies
    • cd68

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-Fr
Rat
IHC-P
Mouse
Rat
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (8)
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 35 kDa.

The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions.

IHC-P (31)
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr (5)
Use a concentration of 0.5 - 1 µg/ml.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 35 kDa.

The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions.

IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Target information above from: UniProt accession P34810 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12514 Mouse
    • Entrez Gene: 287435 Rat
    • SwissProt: P31996 Mouse
    • Unigene: 15819 Mouse
    • Unigene: 12478 Rat
    • Alternative names

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)

      Paraffin-embedded rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous Abreview.

      ab125212 staining CD68 in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA + 10% FCS) for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)

      CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab125212 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    • Western blot - Anti-CD68 antibody (ab125212)
      Western blot - Anti-CD68 antibody (ab125212)
      All lanes : Anti-CD68 antibody (ab125212)

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Mouse spleen tissue lysate
      Lane 3 : RAW264.7 cell lysate

      Predicted band size: 35 kDa
      Additional bands at: 90-100 kDa (possible glycosylated form)



      Western blot analysis of rat and mouse tissue lysates, labelling CD68 with ab125212

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)

      Paraffin-embedded mouse spleen tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry analysis of paraffin-embedded rat liver tissue labeling CD68 with ab125212. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti- CD68 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
       
       
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)Wes, P.D. PLoS One. 2014 Aug 25;9(8):e106050. doi: 10.1371/journal.pone.0106050. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemistry of microglial marker in mouse brain

      Cortical staining for the microglial marker, CD68 increased as a function of age in rTg4510 animals, but remained unchanged in tTA animals. Scale bar, 200 µm, inset scale bar, 50 µm.

      (After Figure 10 A of Wes et al.)

    • Immunohistochemistry (Frozen sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Frozen sections) - Anti-CD68 antibody (ab125212)

      Frozen sectioned rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous Abreview.

      ab125212 staining CD68 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous Abreview
      Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

      Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (undiluted) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (258)

    Publishing research using ab125212? Please let us know so that we can cite the reference in this datasheet.

    ab125212 has been referenced in 258 publications.

    • Zheng L  et al. Inhibition of TIM-4 protects against cerebral ischaemia-reperfusion injury. J Cell Mol Med 24:1276-1285 (2020). PubMed: 31774937
    • Zhou YQ  et al. Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection. EMBO Mol Med 12:e10233 (2020). PubMed: 31782624
    • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
    • Lee JB  et al. Microchannel network hydrogel induced ischemic blood perfusion connection. Nat Commun 11:615 (2020). PubMed: 32001693
    • Sorkin M  et al. Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing. Nat Commun 11:722 (2020). PubMed: 32024825
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 51 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Thomas Vazquez

    Verified customer

    Submitted Feb 25 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph:6.0
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

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    Submitted Aug 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Normal Mouse Liver Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    Normal Mouse Liver Tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
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    Verified customer

    Submitted Apr 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
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    Verified customer

    Submitted Apr 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Apr 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 26 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Common marmoset Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 25 2019

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