Anti-CD68 antibody (ab125212)

Rabbit polyclonal CD68 antibody. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 117 publication(s). Independently reviewed in 38 review(s).

Overview

  • Product name
  • Description
    Rabbit polyclonal to CD68
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CD68 (internal sequence).

  • Positive control
    • WB: Rat and mouse spleen tissue lysate. RAW 264.7 cell lysate. IHC-P: Mouse spleen and skin tissue. IHC-Fr: Rat liver tissue.
  • General notes

      

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 5.61% Trehalose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 35 kDa.

The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Immunohistochemistry of microglial marker

    Cortical staining for the microglial marker, CD68 increased as a function of age in rTg4510 animals, but remained unchanged in tTA animals. Scale bar, 200 µm, inset scale bar, 50 µm.

    (After Figure 10 A of Wes et al)

  • Paraffin-embedded rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

  • Frozen sectioned rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

  • All lanes : Anti-CD68 antibody (ab125212)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : RAW246.7 cell lysate

    Predicted band size: 35 kDa
    Additional bands at: 90-100 kDa (possible glycosylated form)



    Western blot analysis of rat and mouse tissue lysates, labelling CD68 with ab125212

  • Paraffin-embedded mouse spleen tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.

  • Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (undiluted) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

    See Abreview

  • ab125212 staining CD68 in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA + 10% FCS) for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 5% goat serum) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

  • ab125212 staining CD68 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Sun H  et al. Role of the mTOR-FOXO1 pathway in obesity-associated renal tubulointerstitial inflammation. Mol Med Rep 19:1284-1293 (2019). Read more (PubMed: 30535458) »
  • Yew WP  et al. Early treatment with minocycline following stroke in rats improves functional recovery and differentially modifies responses of peri-infarct microglia and astrocytes. J Neuroinflammation 16:6 (2019). Read more (PubMed: 30626393) »
See all 129 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 40 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary murine microglia)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Treatment
1µM LPA for different time points
Specification
Primary murine microglia
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary murine microglia)
Permeabilization
Yes - Triton X
Specification
Primary murine microglia
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Oct 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Epitope retrieval 2 solution (pH=9)
Permeabilization
No
Specification
Skin
Fixative
Paraformaldehyde

Zohar Gavish

Verified customer

Submitted Sep 25 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Tonsil
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 19 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Aug 23 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer, pH 6.0
Permeabilization
Yes - 0.1% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum + 0.3 M Glycine as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Sergi Bayod

Verified customer

Submitted Jun 29 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (Retinal Flat mount)
Specification
Retinal Flat mount

Mr. Tyler Kilburn

Verified customer

Submitted Mar 24 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
kidney
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
kidney
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Rebecca Lilley

Verified customer

Submitted Dec 14 2017

1-10 of 40 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up