Anti-CD68 antibody [C68/684] (ab201340)

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Key features and details

  • Mouse monoclonal [C68/684] to CD68
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD68 antibody [C68/684]
    See all CD68 primary antibodies

  • Description

    Mouse monoclonal [C68/684] to CD68

  • Host species

    Mouse

  • Specificity

    ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey

  • Immunogen

    Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).

  • Positive control

    • IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.

  • General notes

    ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.

  • Clonality

    Monoclonal

  • Clone number

    C68/684

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 1 - 2 µg/ml.

Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes.

Incubate with primary for 30 minutes at RT.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

  • Sequence similarities

    Belongs to the LAMP family.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.

  • Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)
    Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)

    Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)
    Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)

    Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.

  • Western blot - Anti-CD68 antibody [C68/684] (ab201340)
    Western blot - Anti-CD68 antibody [C68/684] (ab201340)
    Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate

    Predicted band size: 37 kDa
    Additional bands at: 110 kDa (possible glycosylated form)

References

Publishing research using ab201340? Please let us know so that we can cite the reference in this datasheet.

ab201340 has been referenced in 20 publications.

  • Li P  et al. Loss of PARP-1 attenuates diabetic arteriosclerotic calcification via Stat1/Runx2 axis. Cell Death Dis 11:22 (2020). PubMed: 31924749
  • Yang HC  et al. C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization. World J Stem Cells 12:152-167 (2020). PubMed: 32184939
  • Wang J  et al. Suberoylanilide hydroxamic acid alleviates orthotopic liver transplantation-induced hepatic ischemia-reperfusion injury by regulating the AKT/GSK3ß/NF-?B and AKT/mTOR pathways in rat Kupffer cells. Int J Mol Med 45:1875-1887 (2020). PubMed: 32236599
  • Kerstetter-Fogle A  et al. Plant Virus-Like Particle In Situ Vaccine for Intracranial Glioma Immunotherapy. Cancers (Basel) 11:N/A (2019). PubMed: 30974896
  • Pakharukova MY  et al. Opisthorchis felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured hamster liver. PLoS One 14:e0216757 (2019). PubMed: 31086416
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [C68/684]

 Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Liver, splen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6,0
Permeabilization
No
Specification
Liver, splen
Fixative
Formaldehyde
Read More

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 09 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [C68/684]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6,0
Permeabilization
No
Specification
Liver
Blocking step
(agent) for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde
Read More

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 02 2017

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