Anti-CD68 antibody [C68/684] (ab201340)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)
  • Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)
  • Western blot - Anti-CD68 antibody [C68/684] (ab201340)

Key features and details

  • Mouse monoclonal [C68/684] to CD68
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD68 antibody [C68/684]
    See all CD68 primary antibodies

  • Description

    Mouse monoclonal [C68/684] to CD68

  • Host species

    Mouse

  • Specificity

    ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey

  • Immunogen

    Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
    Database link: P34810

  • Positive control

    • IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.

  • General notes

    ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.

  • Clonality

    Monoclonal

  • Clone number

    C68/684

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P (2)
Use a concentration of 1 - 2 µg/ml.

Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes.

Incubate with primary for 30 minutes at RT.
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P
Use a concentration of 1 - 2 µg/ml.

Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes.

Incubate with primary for 30 minutes at RT.
ICC/IF
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

  • Sequence similarities

    Belongs to the LAMP family.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.

    • Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)
      Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)

      Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)
      Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)

      Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.

    • Western blot - Anti-CD68 antibody [C68/684] (ab201340)
      Western blot - Anti-CD68 antibody [C68/684] (ab201340)
      Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate

      Predicted band size: 37 kDa
      Additional bands at: 110 kDa (possible glycosylated form)

    References (37)

    Publishing research using ab201340? Please let us know so that we can cite the reference in this datasheet.

    ab201340 has been referenced in 37 publications.

    • Wang ZY  et al. Protein deglycase DJ-1 deficiency induces phenotypic switching in vascular smooth muscle cells and exacerbates atherosclerotic plaque instability. J Cell Mol Med N/A:N/A (2021). PubMed: 33501750
    • Ding S  et al. Macrophage-derived netrin-1 contributes to endometriosis-associated pain. Ann Transl Med 9:29 (2021). PubMed: 33553322
    • Lin QY  et al. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med 11:e374 (2021). PubMed: 33783987
    • Liang Q  et al. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Cell Death Dis 12:336 (2021). PubMed: 33795653
    • Khalifa A  et al. A simple method for implanting free-floating microdevices into the nervous tissue. J Neural Eng 18:N/A (2021). PubMed: 33827069
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [C68/684]

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (Liver, splen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6,0
    Permeabilization
    No
    Specification
    Liver, splen
    Fixative
    Formaldehyde
    Read More

    Dr. Marcelo Pelajo Machado

    Verified customer

    Submitted May 09 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [C68/684]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6,0
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    (agent) for 20 minute(s) · Concentration: 2% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More

    Dr. Marcelo Pelajo Machado

    Verified customer

    Submitted May 02 2017

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