Anti-CD68 antibody [C68/684] (ab201340)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-CD68 antibody [C68/684]
See all CD68 primary antibodies -
DescriptionMouse monoclonal [C68/684] to CD68
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Host speciesMouse
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Specificityab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.
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Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-Fr, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Monkey
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Immunogen
Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
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Positive control
- Human tonsil tissue; Lymph Node and Spleen.
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General notes
ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A/G purified
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Purification notesab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
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ClonalityMonoclonal
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Clone numberC68/684
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa). | |
| IP | Use at 1-2 µg/mg of lysate. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. For 30 minutes at RT. |
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| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For 30 minutes at RT. |
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| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
Target
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FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
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Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
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Sequence similaritiesBelongs to the LAMP family.
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Post-translational
modificationsN- and O-glycosylated. -
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
see all -
Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)](https://www.abcam.com/ps/products/201/ab201340/Images/ab201340-243023-anti-cd68-antibody-c68-684-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.
Protocols
References
This product has been referenced in:
- Zhang X et al. Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy. J Cell Physiol 234:6917-6926 (2019). Read more (PubMed: 30478987) »
- Wu J et al. Protective effects of HTD4010, a Reg3a/PAP-derived peptide, in mouse model of acute pancreatitis via toll-like receptor 4 pathway. Biochem Biophys Res Commun 512:670-677 (2019). Read more (PubMed: 30922566) »
