Anti-CD68 antibody [C68/684] (ab201340)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)](https://www.abcam.com/ps/products/201/ab201340/Images/ab201340-243023-anti-cd68-antibody-c68-684-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)")
Key features and details
- Mouse monoclonal [C68/684] to CD68
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD68 antibody [C68/684]
See all CD68 primary antibodies -
Description
Mouse monoclonal [C68/684] to CD68 -
Host species
Mouse -
Specificity
ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68. -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
Database link: P34810 -
Positive control
- IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.
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General notes
ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
C68/684 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
|
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| Flow Cyt | (1) |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| IHC-P | (2) |
Use a concentration of 1 - 2 µg/ml.
Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa). |
|
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.
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![Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)](https://www.abcam.com/ps/products/201/ab201340/Images/ab201340-396031-anti-cd68-antibody-c68-684-flow-cytometry-u87mg.jpg)
Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)](https://www.abcam.com/ps/products/201/ab201340/Images/ab201340-396034-anti-cd68-antibody-c68-684-immunocytochemistry-u87mg.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.
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![Western blot - Anti-CD68 antibody [C68/684] (ab201340)](https://www.abcam.com/ps/products/201/ab201340/Images/ab201340-396035-anti-cd68-antibody-c68-684-western-blot-spleen-human.jpg)
Western blot - Anti-CD68 antibody [C68/684] (ab201340)Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate
Predicted band size: 37 kDa
Additional bands at: 110 kDa (possible glycosylated form)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab201340 has been referenced in 45 publications.
- Wang ZY et al. Protein deglycase DJ-1 deficiency induces phenotypic switching in vascular smooth muscle cells and exacerbates atherosclerotic plaque instability. J Cell Mol Med N/A:N/A (2021). PubMed: 33501750
- Ding S et al. Macrophage-derived netrin-1 contributes to endometriosis-associated pain. Ann Transl Med 9:29 (2021). PubMed: 33553322
- Lin QY et al. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med 11:e374 (2021). PubMed: 33783987
- Liang Q et al. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Cell Death Dis 12:336 (2021). PubMed: 33795653
- Khalifa A et al. A simple method for implanting free-floating microdevices into the nervous tissue. J Neural Eng 18:N/A (2021). PubMed: 33827069