Overview

  • Product name
    Anti-CD68 antibody [C68/684]
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [C68/684] to CD68
  • Host species
    Mouse
  • Specificity
    ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).

  • Positive control
    • Human tonsil tissue; Lymph Node and Spleen.
  • General notes

    ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Purification notes
    ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
  • Clonality
    Monoclonal
  • Clone number
    C68/684
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
IP Use at 1-2 µg/mg of lysate.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

For 30 minutes at RT.

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For 30 minutes at RT.

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.

References

This product has been referenced in:
  • Zhang X  et al. Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT-1-TREM-1 pathway in diabetic nephropathy. J Cell Physiol 234:6917-6926 (2019). Read more (PubMed: 30478987) »
  • Wu J  et al. Protective effects of HTD4010, a Reg3a/PAP-derived peptide, in mouse model of acute pancreatitis via toll-like receptor 4 pathway. Biochem Biophys Res Commun 512:670-677 (2019). Read more (PubMed: 30922566) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Liver, splen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6,0
Permeabilization
No
Specification
Liver, splen
Fixative
Formaldehyde

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 09 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6,0
Permeabilization
No
Specification
Liver
Blocking step
(agent) for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 02 2017

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