Product nameAnti-CD68 antibody [ED1]
See all CD68 primary antibodies
DescriptionMouse monoclonal [ED1] to CD68
SpecificityAb31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, WB, RIA, IHC-FrFl, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Tissue, cells or virus corresponding to CD68. Rat spleen cell.
- Flow Cyt: Rat peritoneal macrophages. IHC-P: Rat liver and kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 488) (ab201844)
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 647) (ab201845)
- Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
- Anti-CD68 antibody [FA-11] (ab53444)
- Anti-CD11b antibody [M1/70] (ab8878)
- Anti-F4/80 antibody [F4/80] (ab90247)
Our Abpromise guarantee covers the use of ab31630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
|IHC-Fr||Use at an assay dependent concentration.
Fixation with acetone is recommended.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.|
|RIA||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 25374513|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Alternatively protein digestion with trypsin or pronase prior to IHC protocol could be preformed.|
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Pulmonary immunochemical staining in rats with selective COX inhibition.
CD68: SC-560 (Top left panel) diminished CD-68 positive macrophages (green arrow indicating brown cells) accumulation compared to control (Top right panel) and celecoxib (Bottom panel) groups (magnification 200x).
Rat lung tissue were dissected free and fixed in 10% formalin solution.
ab31630 used at a 1:200 dilution.
Effect of a pharmacological inhibition of a T cell function on IA progression.
F344/Jcl rats were subjected to IA (intracranial aneurysms) induction and, at 14th day after the induction, slices from IA specimen induced at ACA-OA bifurcation were prepared for further analyses (IHC-Fr sections).
Administration of Cyclosporine A was started 1 day before the induction and lasted for a whole experimental period (15 mg/kg, once a day). Systemic blood pressure was evaluated before sacrifice without any anesthesia.
Number of infiltrated macrophages in IA lesions was evaluated after immunostaining for a macrophage marker CD68 (per 12,100 μm2). Data represents mean ± SEM. Representative images of immunostaining for CD68 (ab31630, green) and α-smooth muscle actin (α-SMA, red) and of nuclear staining DAPI (blue) are shown in lower left panels. Bar, 20 μm.
ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.
Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm.
ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.
This product has been referenced in:
- Bezhaeva T et al. Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease. Biomaterials 194:47-56 (2019). Read more (PubMed: 30580195) »
- Kim EY et al. Trpc6 inactivation confers protection in a model of severe nephrosis in rats. J Mol Med (Berl) 96:631-644 (2018). Read more (PubMed: 29785489) »