Overview

  • Product name
    Anti-CD68 antibody [ED1]
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [ED1] to CD68
  • Host species
    Mouse
  • Specificity
    Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WB, RIA, IHC-FrFl, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to CD68. Rat spleen cell.

  • Positive control
    • Flow Cyt: Rat peritoneal macrophages. IHC-P: Rat liver and kidney tissue.
  • General notes
    This product should be stored undiluted.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    ED1
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Fixation with acetone is recommended.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
RIA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25374513
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Alternatively protein digestion with trypsin or pronase prior to IHC protocol could be preformed.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Effect of a pharmacological inhibition of a T cell function on IA progression.

    F344/Jcl rats were subjected to IA (intracranial aneurysms) induction and, at 14th day after the induction, slices from IA specimen induced at ACA-OA bifurcation were prepared for further analyses (IHC-Fr sections).

    Administration of Cyclosporine A was started 1 day before the induction and lasted for a whole experimental period (15 mg/kg, once a day). Systemic blood pressure was evaluated before sacrifice without any anesthesia.

    Number of infiltrated macrophages in IA lesions was evaluated after immunostaining for a macrophage marker CD68 (per 12,100 μm2). Data represents mean ± SEM. Representative images of immunostaining for CD68 (ab31630, green) and α-smooth muscle actin (α-SMA, red) and of nuclear staining DAPI (blue) are shown in lower left panels. Bar, 20 μm.

  • Pulmonary immunochemical staining in rats with selective COX inhibition.

    CD68: SC-560 (Top left panel) diminished CD-68 positive macrophages (green arrow indicating brown cells) accumulation compared to control (Top right panel) and celecoxib (Bottom panel) groups (magnification 200x).

    Rat lung tissue were dissected free and fixed in 10% formalin solution.

    ab31630 used at a 1:200 dilution.

  • ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm.

  • ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

References

This product has been referenced in:
  • Kuismanen K  et al. Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model. Stem Cells Transl Med 7:295-304 (2018). IHC-P ; Rat . Read more (PubMed: 29383878) »
  • Yu ZM  et al. Mechanism of Chronic Stress-induced Reduced Atherosclerotic Medial Area and Increased Plaque Instability in Rabbit Models of Chronic Stress. Chin Med J (Engl) 131:161-170 (2018). Read more (PubMed: 29336364) »
See all 107 Publications for this product

Customer reviews and Q&As

1-10 of 40 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Dr. Alexandre Magno

Verified customer

Submitted Jul 31 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Tumor)
Permeabilization
Yes - 100% Acetone
Specification
Tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Aug 02 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Lung)
Permeabilization
Yes - 80% Acetone
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 02 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 19 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Injured spinal cord)
Antigen retrieval step
None
Permeabilization
No
Specification
Injured spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Microglia)
Permeabilization
Yes - Triton X100
Specification
Microglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Ray

Verified customer

Submitted Feb 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citrate buffer, pH6.0
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2014

1-10 of 40 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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