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Anti-CD68 antibody [ED1] (ab31630)

Reviews (20)Q&A (4)References (301)
Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)

    Key features and details

    • Mouse monoclonal [ED1] to CD68
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Rat
    • Isotype: IgG1

    Overview

    • Product name

      Anti-CD68 antibody [ED1]
      See all CD68 primary antibodies

    • Description

      Mouse monoclonal [ED1] to CD68

    • Host species

      Mouse

    • Specificity

      Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

    • Tested applications

      Suitable for: Flow Cyt (Intra)more details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      Tissue, cells or virus corresponding to Rat CD68. Rat spleen cell.

    • Positive control

      • Flow Cyt (Intra): Rat peritoneal macrophages.

    • General notes

      This product should be stored undiluted.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    1/50 - 1/100.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    Notes
    Flow Cyt (Intra)
    1/50 - 1/100.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • Sequence similarities

      Belongs to the LAMP family.

    • Post-translational
      modifications

      N- and O-glycosylated.

    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)
        Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)

        Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm. 

         

         

      References (301)

      Publishing research using ab31630? Please let us know so that we can cite the reference in this datasheet.

      ab31630 has been referenced in 301 publications.

      • Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
      • Bandoni RL  et al. Cholinergic stimulation with pyridostigmine modulates a heart-spleen axis after acute myocardial infarction in spontaneous hypertensive rats. Sci Rep 11:9563 (2021). PubMed: 33953291
      • Chen ML  et al. Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy. Theranostics 11:2395-2409 (2021). PubMed: 33500732
      • Irastorza-Lorenzo A  et al. Evaluation of Marine Agarose Biomaterials for Tissue Engineering Applications. Int J Mol Sci 22:N/A (2021). PubMed: 33672027
      • Yu Q  et al. Dexmedetomidine suppresses the development of abdominal aortic aneurysm by downregulating the mircoRNA-21/PDCD 4 axis. Int J Mol Med 47:N/A (2021). PubMed: 33786608
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      1-4 of 4 Q&A

      Question

      I am interested in using anti-CD68 antibody [ED1] (ab31630) in mouse brain paraffin embedded sections, but am wondering if it is necessary to use a mouse-on-mouse kit for this to work.

      Read More
      Answer

      Since the sample and the host species of this primary antibody is the same (mouse), there is a chance for getting high non-specific background signal.
      You may be able to work around this problem if you apply ABC signal enhancing system which requires Biotin-conjugated secondary antibody.

      Read More

      Question

      The description indicates for this CD68 antibody says that "Ab31630 recognises a single chain glycoprotein of 110kD," yet the predicted size is 37kDa. Is this correct? I am only detecting a 110kDa band in rat microglia by western blot. (I loaded 8ug/lane, 5% milk TBST 1hr block, ECL detection.)

      Read More
      Answer

      Thank you for your inquiry.

      I can confirm that prediction of the 110 kDa is based on literature references as follows:
      1. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J, Neuro-chem. 88:1335-1344.
      2. Kim H, et al. Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats. J
      Vet Sci. 2007 Dec;8(4):323-7. PubMed PMID: 17993744
      3. http://www.ncbi.nlm.nih.gov/pubmed/7821959

      These references show a band of 110kDa (ED1 is the rat homologue of human CD68). Looking at the available literature for Rat CD68 it appears that is accepted that it can appear on a Western blot across a range of 30-110kDa depending on conditions and experimental setup. This is most likely due to doublet and triplet formation although I have been unable to confirm this with referenced material.

      I hope this information is helpful for you. Please do not hesitate to contact me again if you need more information.

      Read More

      Question

      Hi,   I am interested in the  Anti-CD68 antibody [ED1] (ab31630).  I would be using it on mouse brain tissue and although your online data sheet mentions that it reacts with both mouse and rat, I have not seen a published report on mouse tissue, only rat. I just want to confirm that it does work on mouse before i purchase. Thank you for any information you can offer.  

      Read More
      Answer

      Thank you for your enquiry. I can confirm ab31630 does work on mouse tissue. I hope this is helpful. Please contact me again if you have any further questions.

      Read More

      Question

      Can you tell me the source of ”ED1 is the rat homologue of human CD68” statement?

      Read More
      Answer

      Thank you for your enquiry. The statement was mentioned in the following publications. 1) Feridex Preloading Permits Tracking of CNS-Resident Macrophages Following Transient Middle Cerebral Artery Occlusion. J Cereb Blood Flow Metab. 2009 July; 29(7): 1229–1239. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2744340/ 2) The PPAR gamma agonist Pioglitazone improves anatomical and locomotor recovery after rodent spinal cord injury. Experimental Neurology Volume 205, Issue 2, June 2007, Pages 396-406

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