Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [ED1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Alexa 647 donkey anti-mouse secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647

Additional data

Additional Notes
Green was the false color used to represent CD68 positive cells detected by Alexa 647 conjugated donkey anti-mouse secondary antibody.

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Submitted Apr 17 2017

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