Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0,2% triton 100x+4%BSA
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Se
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Take in mind that not always you will see a reactivity because of the nature of the CD68 protein. Its expression depends on the levels of inflammation.
Image info: In green anti-Iba1 with Alexa Fluor 488 in red anti-CD68 with Alexa Fluor 568
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alexandre Magno
Verified customer
Submitted Jul 31 2018