Recombinant Anti-CD68 antibody [EPR20545] - Goat IgG (ab289671)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)](https://www.abcam.com/ps/products/289/ab289671/Images/ab289671-481987-anti-CD68-antibody-epr20545-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [EPR20545] to CD68 - Goat IgG
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD68 antibody [EPR20545] - Goat IgG
See all CD68 primary antibodies -
Description
Goat monoclonal [EPR20545] to CD68 - Goat IgG -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra) or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human tonsil and liver tissue lysates; THP-1 whole cell lysate. IHC-P: Human liver and tonsil tissue.
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General notes
This goat antibody has been engineered from a RabMAb parent antibody (ab213363). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20545 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
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| Notes |
|---|
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD68 with ab289671 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in human tonsil. The section was incubated with ab289671 for 30 mins at room temperature and followed by goat specific IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)](https://www.abcam.com/ps/products/289/ab289671/Images/ab289671-481985-anti-CD68-antibody-epr20545-western-blot-human.jpg)
Western blot - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)All lanes : Anti-CD68 antibody [EPR20545] - Goat IgG (ab289671) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit anti-Goat IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times - Lanes 1-2: 3 minutes; Lane 3: 37 seconds.
The observed molecular weight is consistent with the literature (PMID:18405323, 11739566, 16710801).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)](https://www.abcam.com/ps/products/289/ab289671/Images/ab289671-481986-anti-CD68-antibody-epr20545-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric (ab289671)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD68 with ab289671 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kuffer cells in human liver. The section was incubated with ab289671 for 30 mins at room temperature and followed by goat specific IgG antibody (ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-CD68 antibody [EPR20545] - Goat IgG (ab289671)](https://www.abcam.com/ps/products/289/ab289671/Images/ab289671-482000-benefitsofrecombinantantibodies.jpg)
Anti-CD68 antibody [EPR20545] - Goat IgG (ab289671)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289671 has not yet been referenced specifically in any publications.