Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [FA-11]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 (Ventana mRcc)
Permeabilization
No
Specification
Lung
Fixative
10% Neutral Buffered Formalin

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: DAKO diluent with background reducing
Dilution
1/500

Secondary antibody

Concentration
3 µg/ml
Name
Non-Abcam antibody was used: DAKO Biotinylated Rabbit anti rat E0468
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Tissue was fixed in 10% NBF for 24 hours. Dewaxing was carried out offline through a xylene/ alcohol series Staining procedure was carried out on Ventana Discovery XT using DABMap detection kit Primary antibody incubation was carried out using the no-heat setting. Secondary antibody was incubated at 37oC for 32 min.

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Submitted Oct 18 2012

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