12 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: DAKO diluent with background reducing
Non-Abcam antibody was used: DAKO Biotinylated Rabbit anti rat E0468 Host species: Rabbit Clonality: Polyclonal Conjugation: Biotin
Tissue was fixed in 10% NBF for 24 hours.
Dewaxing was carried out offline through a xylene/ alcohol series
Staining procedure was carried out on Ventana Discovery XT using DABMap detection kit
Primary antibody incubation was carried out using the no-heat setting.
Secondary antibody was incubated at 37oC for 32 min.
Abcam user community
Submitted Oct 18 2012
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