Western blot abreview for Anti-CD68 antibody [FA-11]

Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Raw264.7)
Specification
Raw264.7
Treatment
100 ng/ml LPS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rat secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 37 kDa
Exposure
1 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Aug 19 2014

Sign up