Overview

  • Product name

    Anti-CD68 antibody [FA-11] - BSA and Azide free
    See all CD68 primary antibodies
  • Description

    Rat monoclonal [FA-11] to CD68 - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD68.

  • Positive control

    • IHC-Fr: Mouse lung, spleen and heart tissue sections; ICC/IF: RAW 246.7 cell line. Flow Cyt: Mouse peritoneal macrophages.
  • General notes

    Ab23796 is a PBS only version of ab53444.

    Although some customers have had success with this antibody in IHC-P, we are unable to obtain positive results in this application and so cannot recommend it for IHC-P. We batch test the antibody in IHC-Fr.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    FA-11
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 5 µg/ml.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • ab53444 stained in RAW 246.7 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53444 at 1µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150165, Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), preadsorbed (colored red), both used at 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).

  • Formaldehyde-fixed, frozen mouse lung tissue sections stained for CD68 using ab53444 at a 1/250 dilution in immunohistochemical analysis. Tissue sections were blocked using 1% BSA as a blocking agent for 10 minutes at 21°C. Primary antibody was incubated for 2 hours at 21°C. Secondary antibody was a biotin-conjugated goat anti-rat IgG at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).

  • IHC image of CD68 staining in mouse lung frozen tissue section. The section was incubated with ab53444, 0.1µg/ml, overnight at 4C. A goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).

  • ab53444 staining CD68 in mouse spleen tissue by Immunohistochemistry (Frozen sections). Antibody was detected with HRP-conjugated Goat anti-Rat IgG, showing staining in the red pulp.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).

  • Staining of permeabilised mouse peritoneal macrophages with Rat anti mouse CD68 (ab53444).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).

References

ab237968 has not yet been referenced specifically in any publications.

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