Recombinant Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
![Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)](https://www.abcam.com/ps/products/237/ab237968/Images/ab237968-485184-anticd68antibodyfa11immunohistochemistryfrozenlungmouse.jpg "Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [FA-11] to CD68 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD68 antibody [FA-11] - BSA and Azide free
See all CD68 primary antibodies -
Description
Rat monoclonal [FA-11] to CD68 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: Mouse lung, spleen and heart tissue sections; ICC/IF: Raw264.7 (LPS treated) cells. Flow Cyt: Mouse peritoneal macrophages.
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General notes
ab23796 is the carrier-free version of ab53444.
Although some customers have had success with this antibody in IHC-P, we are unable to obtain positive results in this application and so cannot recommend it for IHC-P. We batch test the antibody in IHC-Fr.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
FA-11 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
- Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
- Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
- HRP Anti-CD68 antibody [FA-11] (ab216526)
- PE Anti-CD68 antibody [FA-11] (ab216701)
- PerCP Anti-CD68 antibody [FA-11] (ab220509)
- APC Anti-CD68 antibody [FA-11] (ab221251)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-Fr |
Use a concentration of 0.1 - 5 µg/ml.
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| Notes |
|---|
|
Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-Fr
Use a concentration of 0.1 - 5 µg/ml. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 12514 Mouse
- SwissProt: P31996 Mouse
- Unigene: 15819 Mouse
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
This data was developed using ab53444, the same antibody clone in a different buffer formulation.
IHC image of CD68 staining in a section of frozen mouse lung performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab53444, 0.1ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)](https://www.abcam.com/ps/products/237/ab237968/Images/ab237968-6-anti-cd68-fa11-bsa-and-azide-free-antibody-immunocytochemistry-raw2647-lps-treated-mouse.jpg)
Immunocytochemistry - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)ab237968 staining CD68 in Raw264.7 (LPS treated) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab237968 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)](https://www.abcam.com/ps/products/237/ab237968/Images/ab237968-354249-anti-cd68-antibody-fa11-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)ab53444 staining CD68 in mouse spleen tissue by Immunohistochemistry (Frozen sections). Antibody was detected with HRP-conjugated Goat anti-Rat IgG, showing staining in the red pulp.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab53444).
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![Flow Cytometry - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)](https://www.abcam.com/ps/products/237/ab237968/Images/ab237968-354247-anti-cd68-antibody-fa11-flow-cytometry-peritoneal-macrophages-mouse.jpg)
Flow Cytometry - Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab237968 has been referenced in 1 publication.
- Shi W et al. Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis. Biosci Rep 41:N/A (2021). PubMed: 33969376
