Key features and details
- Rat monoclonal [FA-11] to CD68 (HRP)
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2a
Product nameAnti-CD68 antibody [FA-11] (HRP)
See all CD68 primary antibodies
DescriptionRat monoclonal [FA-11] to CD68 (HRP)
SpecificityDetects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse CD68.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 405) (ab199571)
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 488) (ab201844)
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 647) (ab201845)
- Anti-CD68 antibody [FA-11] (PE) (ab216701)
- Anti-CD68 antibody [FA-11] (PerCP) (ab220509)
- Anti-CD68 antibody [FA-11] (APC) (ab221251)
- Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
- Anti-CD68 antibody [FA-11] (ab53444)
Our Abpromise guarantee covers the use of ab216526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.|
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
All lanes : Anti-CD68 antibody [FA-11] (HRP) (ab216526) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 LPS treated (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Additional bands at: 100 kDa (possible glycosylated form)
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab216526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab216526 has not yet been referenced specifically in any publications.