Key features and details
- Mouse monoclonal [KP1 + C68/684] to CD68
- Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD68 antibody [KP1 + C68/684]
See all CD68 primary antibodies
DescriptionMouse monoclonal [KP1 + C68/684] to CD68
Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD68. Subcellular fraction of Human alveolar macrophages.
- Tonsil, Lymph Node or Spleen tissues.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
PurityProtein G purified
Clone numberKP1 + C68/684
Light chain typekappa
Our Abpromise guarantee covers the use of ab199000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.|
|IP||Use at 1-2 µg/mg of lysate.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
ab199000 has been referenced in 2 publications.
- Lamano JB et al. Glioblastoma-Derived IL6 Induces Immunosuppressive Peripheral Myeloid Cell PD-L1 and Promotes Tumor Growth. Clin Cancer Res N/A:N/A (2019). PubMed: 30824583
- Li YD et al. Systemic and local immunosuppression in patients with high-grade meningiomas. Cancer Immunol Immunother 68:999-1009 (2019). PubMed: 31030234