Anti-CD68 antibody [KP1 + C68/684] (ab199000)
Key features and details
- Mouse monoclonal [KP1 + C68/684] to CD68
- Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD68 antibody [KP1 + C68/684]
See all CD68 primary antibodies -
Description
Mouse monoclonal [KP1 + C68/684] to CD68 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD68. Subcellular fraction of Human alveolar macrophages.
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Positive control
- Tonsil, Lymph Node or Spleen tissues.
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General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KP1 + C68/684 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab199000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa. | |
| IP | Use at 1-2 µg/mg of lysate. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes |
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| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (ab199000)](https://www.abcam.com/ps/products/199/ab199000/Images/ab199000-240247-anti-cd68-antibody-kp1-c68-684-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (ab199000)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab199000 at 1µg/ml (10X).
Protocols
Datasheets and documents
References
ab199000 has been referenced in 2 publications.
- Lamano JB et al. Glioblastoma-Derived IL6 Induces Immunosuppressive Peripheral Myeloid Cell PD-L1 and Promotes Tumor Growth. Clin Cancer Res N/A:N/A (2019). PubMed: 30824583
- Li YD et al. Systemic and local immunosuppression in patients with high-grade meningiomas. Cancer Immunol Immunother 68:999-1009 (2019). PubMed: 31030234
