Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free (ab213099)

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Overview

  • Product name

    Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free
    See all CD68 primary antibodies

  • Description

    Mouse monoclonal [KP1 + C68/684] to CD68 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Cat, Human, Monkey

  • Immunogen

    This product was produced with the following immunogens:
    Tissue, cells or virus corresponding to Human CD68. Subcellular fraction of Human alveolar macrophages (clone KP1)
    Database link: P34810

    Recombinant full length protein corresponding to Human CD68. (clone C68/684)
    Database link: P34810

  • Positive control

    • Human histiocytoma, Human tonsil, lymph node and spleen tissues.

  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from bioreactor concentrate.

  • Clonality

    Monoclonal

  • Clone number

    KP1 + C68/684

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at room temperature.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

  • Sequence similarities

    Belongs to the LAMP family.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free (ab213099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free (ab213099)

      Immunohistochemical analysis of formalin fixed paraffin-embedded Human histiocytoma tissue labeling CD68 with ab213099 at 0.5 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free (ab213099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] - BSA and Azide free (ab213099)

      Immunohistochemical analysis of formalin fixed paraffin-embedded Human tonsil tissue labeling CD68 with ab213099 at 0.5 µg/mL.

    References

    ab213099 has not yet been referenced specifically in any publications.

    Publishing research using ab213099? Please let us know so that we can cite the reference in this datasheet.

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