Recombinant Anti-CD68 antibody [KP1] (ab955)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KP1] to CD68
- Suitable for: ICC/IF, WB, IHC-P, Mass Cytometry
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD68 antibody [KP1]
See all CD68 primary antibodies -
Description
Mouse monoclonal [KP1] to CD68 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Mass Cytometrymore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human tonsil and liver tissues; ICC/IF: THP-1 cells; WB: Human spleen lysate, THP-1 whole cell lysate IMC: Human tonsil tissue
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General notes
Anti CD68 antibody (ab955) is reccomended for IHC on human samples but is not reccomended for mouse & rat samples.
This product has switched from a hybridoma to recombinant production method on 21st September 2020. The concentration listed relates to the purified version, please contact Scientific Support for unpurified concentration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KP1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (5) |
1/50.
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WB | (1) |
1/1000. Predicted molecular weight: 37 kDa.
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IHC-P | (30) |
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Mass Cytometry |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/50. |
WB
1/1000. Predicted molecular weight: 37 kDa. |
IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Mass Cytometry
Use at an assay dependent concentration. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Lanes 1 & 3 : Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution
Lanes 2 & 4 : Anti-CD68 antibody [RM1031] (ab303565) at 1/1000 dilution
Lanes 1-2 : Human spleen tissue lysate
Lanes 3-4 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST
Exposure time: Lane1: 60 seconds; Lane2: 5 seconds; Lane3: 180 seconds; Lane4: 7 seconds
ab303565 works better than ab955 in western blot testing.
We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using ab955 in western blot.
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Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD68 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in THP-1 cells
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).
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ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
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ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (527)
ab955 has been referenced in 527 publications.
- Shen H et al. Synergistically targeting synovium STING pathway for rheumatoid arthritis treatment. Bioact Mater 24:37-53 (2023). PubMed: 36582350
- Zhang H et al. CU06-1004 enhances vascular integrity and improves cardiac remodeling by suppressing edema and inflammation in myocardial ischemia-reperfusion injury. Exp Mol Med 54:23-34 (2022). PubMed: 34997212
- Li B et al. RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice. Elife 11:N/A (2022). PubMed: 35001873
- Zhang SS et al. Long-term running exercise improves cognitive function and promotes microglial glucose metabolism and morphological plasticity in the hippocampus of APP/PS1 mice. J Neuroinflammation 19:34 (2022). PubMed: 35123512
- Wang X et al. Inhibition of Integrin αvβ6 Activation of TGF-β Attenuates Tendinopathy. Adv Sci (Weinh) 9:e2104469 (2022). PubMed: 35166070