Recombinant Anti-CD68 antibody [KP1] (ab955)
![Western blot - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-414959-AntiCD68antibodyWesternblot.jpg "Western blot - Anti-CD68 antibody [KP1] (ab955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KP1] to CD68
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD68 antibody [KP1]
See all CD68 primary antibodies -
Description
Mouse monoclonal [KP1] to CD68 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
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Positive control
- IHC-P: human tonsil and liver tissues; ICC: THP-1 cells; WB: Human spleen lysate.
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General notes
Anti CD68 antibody (ab955) is reccomended for IHC on human samples but is not reccomended for mouse & rat samples.
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KP1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 37 kDa.
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| ICC |
1/50.
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| IHC-P | (29) |
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 37 kDa. |
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ICC
1/50. |
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IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Western blot - Anti-CD68 antibody [KP1] (ab955)Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution + Human spleen lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG(H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsThe observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).
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![Immunocytochemistry - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-414752-AntiCD68antibodyImmunocytochemistry.jpg)
Immunocytochemistry - Anti-CD68 antibody [KP1] (ab955)Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD28 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in THP-1 cells
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-414750-AntiCD68antibodyImmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-414749-AntiCD68antibodyImmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-3836-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-3837-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreviewab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
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![Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-18-benefits-of-recombinant-antibodies.png)
Anti-CD68 antibody [KP1] (ab955)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (337)
ab955 has been referenced in 337 publications.
- Li L et al. XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol 235:281-293 (2020). PubMed: 31215024
- Wang J et al. Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer. Cancer Sci 111:219-228 (2020). PubMed: 31729088
- Liu X et al. Primary Alcohol-Activated Human and Mouse Hepatic Stellate Cells Share Similarities in Gene-Expression Profiles. Hepatol Commun 4:606-626 (2020). PubMed: 32258954
- McCormick A et al. Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy Hypertens 20:108-110 (2020). PubMed: 32278308
- Dan H et al. RACK1 promotes cancer progression by increasing the M2/M1 macrophage ratio via the NF-?B pathway in oral squamous cell carcinoma. Mol Oncol 14:795-807 (2020). PubMed: 31997535