Anti-CD68 antibody [KP1] (ab955)
- Datasheet
- References (245)
- Protocols
Overview
-
Product name
Anti-CD68 antibody [KP1]
See all CD68 primary antibodies -
Description
Mouse monoclonal [KP1] to CD68 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
-
Positive control
- IHC-P: human tonsil, spleen, liver and ulcerated oral tissues; ICC/ IF: human lung cancer cells.
-
General notes
IHC is recommended on human samples. IHC is not recommended on mouse and rat samples.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, Water -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
KP1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Antibody Diluent
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18804859 | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IHC is recommended on human samples. IHC is not recommended on mouse and rat samples. Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water. In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT. |
|
| IHC-Fr | Use at an assay dependent concentration. IHC is recommended on human samples. IHC is not recommended on mouse and rat samples. |
Target
-
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
see all -
Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-252273-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemical analysis of tonsil labeling CD68 with ab955.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-263273-anti-cd68-antibody-kp1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)Yuan Z et al., PLoS One 9:e94241 (2014)., doi: 10.1371/journal.pone.0094241ab955 staining CD68 in human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were then incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-263251-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image is courtesy of an anonymous AbReviewab955 staining CD68 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit polymer was used as the secondary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-3836-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-284914-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemical analysis of tonsil labeling CD68 with ab955.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.com/ps/products/0/ab955/Images/ab955-3837-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreviewab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
References
This product has been referenced in:
- Li L et al. XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol 235:281-293 (2020). Read more (PubMed: 31215024) »
- Shafiq M et al. In situ blood vessel regeneration using neuropeptide substance P-conjugated small-diameter vascular grafts. J Biomed Mater Res B Appl Biomater 107:1669-1683 (2019). Read more (PubMed: 30315717) »
