Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver tissue)
Specification
Liver tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST + 5% normal serum
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 568 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/400
Additional data
Additional Notes
Paraffin embedded (IHC-P) section. Excellent antibody, it detected macrophages/kupffer cells within the expected areas of the liver.
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Submitted Jan 05 2011