Recombinant

Recombinant Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

Overview

  • Product name

    Anti-CD68 antibody [KP1] - BSA and Azide free
    See all CD68 primary antibodies
  • Description

    Mouse monoclonal [KP1] to CD68 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human fetal spleen lysate. IHC-P: Human tonsil and iver tissue. ICC/IF: THP-1 cells.
  • General notes

    ab233172 is a PBS-only buffer format of ab955.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    KP1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IHC-P 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Images

    • Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-CD68 antibody [KP1]. ab233172 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

      Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD68 with ab233172 at 1/3000 dilution, followed by Goat Anti-mouse IgG H&L (HRP) ready to use. Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-mouse IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD68 with ab233172 at 1/3000 dilution, followed by Goat Anti-mouse IgG H&L (HRP) ready to use. Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-mouse IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling CD68 with ab233172 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Recombinant Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution.

       

    • Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172) at 1/1000 dilution + Human fetal spleen lysate at 20 µg/ml

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Predicted band size: 37 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      The observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).

    References

    ab233172 has not yet been referenced specifically in any publications.

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