Overview

  • Product name

    Anti-CD68 antibody [PG-M1]
    See all CD68 primary antibodies
  • Description

    Mouse monoclonal [PG-M1] to CD68
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Gaucher cells

  • Positive control

    • IHC-P: Human tonsil and granulomatous inflammatory tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    PG-M1
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
  • Application notes
    Is unsuitable for ICC or IHC-Fr.
  • Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Images

    • ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    • Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.

      See Abreview

    References

    This product has been referenced in:

    • Waller R  et al. Iba-1-/CD68+ microglia are a prominent feature of age-associated deep subcortical white matter lesions. PLoS One 14:e0210888 (2019). Read more (PubMed: 30682074) »
    • Pratt D  et al. Programmed Death Ligand 1 Is a Negative Prognostic Marker in Recurrent Isocitrate Dehydrogenase-Wildtype Glioblastoma. Neurosurgery N/A:N/A (2018). IHC-P . Read more (PubMed: 30011045) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your inquiry.

    I apologise for the delay. I had to wait for an answer from the source of these two products.

    I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

    They also contain 0.05% sodium azide which might interfere with the conjugation reaction.

    I hope this information is helpful and wish you a good weekend.

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (granulomatous inflammatory tissue)
    Specification
    granulomatous inflammatory tissue
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Pepsin
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 21 2008

    Answer

    Thank you for your enquiry regarding ab783. We have received feedback from the source of the antibody telling us that "this CD68 is specific against macrophages only" unfortunately. I would like to recommend looking at the following page from our website to help you find markers of each cell population: http://abcam2/index.html?pageconfig=resource&rid=295&pid=7 I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

    Read More

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