Anti-CD68 antibody [PG-M1], prediluted (ab844)

Abreviews (1)Q&A (1)Specific References (1)

Overview

  • Product name
    Anti-CD68 antibody [PG-M1], prediluted
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [PG-M1] to CD68, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were immunized with Gaucher’s cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    PG-M1
  • Myeloma
    unknown
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
    This antibody is not recommended for frozen sections or cell smears.

    • Target

    • Function
      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity
      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities
      Belongs to the LAMP family.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localization
      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

      References

      This product has been referenced in:
      • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . Read more (PubMed: 24714202) »

      See 1 Publication for this product

      Publishing research using ab844? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      2 reviews or Q&A

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1], prediluted

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Spleen)
      Specification
      Spleen
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
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      Username

      Mr. Carl Hobbs

      Verified customer

      Submitted Sep 06 2012

      Telefon call:

      Wants to label ab783 or ab844 with AF488,
      What is in the buffer of ab844? Will it interfere with the labeling reaction, what is the concentration?

      What is the concentration of ab783?

      Thank you for your inquiry.

      I apologise for the delay. I had to wait for an answer from the source of these two products.

      I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

      Th...

      Read More

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