Anti-CD68 antibody [PG-M1], prediluted (ab844)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)

    Key features and details

    • Mouse monoclonal [PG-M1] to CD68, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG3

    Overview

    • Product name

      Anti-CD68 antibody [PG-M1], prediluted
      See all CD68 primary antibodies

    • Description

      Mouse monoclonal [PG-M1] to CD68, prediluted

    • Host species

      Mouse

    • Specificity

      This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.

    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data

    • Immunogen

      BALB/C mice were immunized with Gaucher’s cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.

    • Storage buffer

      Preservative: 0.05% Sodium azide

      Inert stabilizer
    • Concentration information loading...

    • Clonality

      Monoclonal

    • Clone number

      PG-M1

    • Myeloma

      unknown

    • Isotype

      IgG3

    • Light chain type

      kappa

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab844 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P (1)
    Notes
  • Application notes
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
    This antibody is not recommended for frozen sections or cell smears.

    • Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • Sequence similarities

      Belongs to the LAMP family.

    • Post-translational
      modifications

      N- and O-glycosylated.

    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

      References (1)

      Publishing research using ab844? Please let us know so that we can cite the reference in this datasheet.

      ab844 has been referenced in 1 publication.

      • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . PubMed: 24714202

      Customer reviews and Q&As

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1], prediluted

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Spleen)
      Specification
      Spleen
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Sep 06 2012

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