Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP251] to CD68
- Suitable for: mIHC, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-CD68 antibody [SP251]
See all CD68 primary antibodies
DescriptionRabbit monoclonal [SP251] to CD68
Tested applicationsSuitable for: mIHC, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD68 aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P34810
- IHC-P: Human tonsilFlow Cyt: Raji cells.
FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab192847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|mIHC||Use at an assay dependent concentration.|
Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Incubate with primary antibody for 10 minutes at room temperature.
Recommend Hu species. IHC result showed staining on the macrophages in the human tissues such as tonsil, spleen and colon. The mouse and rat tissues showed no staining.
Incubate with primary antibody for 30 minutes at 4 °C.
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).
Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.
Sodium citrate antigen retrieval (Leica ER1, pH6.0, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.
DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD68 with ab192847 at 1/100 dilution (0.184 μg/ml) (incubated for 10 minutes at room temperature). Heat mediated antigen retrieval was performed with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Negative control: PBS instead of the primary antibody.
Positive staining on the macrophages in human tonsil, performed on a Leica Biosystems BOND® RX instrument.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD68 using ab192847 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD68 with ab192847 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab192847 has not yet been referenced specifically in any publications.