Recombinant
RabMAb

Recombinant Anti-CD68 antibody [SP251], prediluted (ab228185)

Overview

  • Product name

    Anti-CD68 antibody [SP251], prediluted
    See all CD68 primary antibodies
  • Description

    Rabbit monoclonal [SP251] to CD68, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD68 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: P34810

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

  • Form

    Prediluted
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: Tris buffered saline, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP251
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1.

Ready to use.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD68 using ab228185 in immunohistochemical analysis.

References

ab228185 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab228185.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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