Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP251] to CD68, prediluted
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-CD68 antibody [SP251], prediluted
See all CD68 primary antibodies
DescriptionRabbit monoclonal [SP251] to CD68, prediluted
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD68 aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P34810
- IHC-P: Human tonsil tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab228185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Ready to use.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Primary antibody incubation for 10 minutes at room temperature.
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228185 has not yet been referenced specifically in any publications.