Key features and details
- Mouse monoclonal [Y1/82A] to CD68
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-CD68 antibody [Y1/82A]
See all CD68 primary antibodies
DescriptionMouse monoclonal [Y1/82A] to CD68
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Normal lymphocytes stimulated with phytohaemagluttin/Freund's adjuvant.
- Human peripheral blood monocytes for Flow cytometry.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purification notesPurified by affinity chromatography.
Light chain typekappa
Our Abpromise guarantee covers the use of ab134350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.125µg for 106 cells.
in 100 µl volume.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Intracellular immunofluorescent staining with flow cytometric analysis labelling CD68 in Human peripheral blood monocytes at 0.125 µg per million cells in 100 µl volume, intracellularly stained by ab134351.
ab134350 has not yet been referenced specifically in any publications.